Next Article in Journal
A Forensic Genomics Approach for the Identification of Sister Marija Crucifiksa Kozulić
Previous Article in Journal
Stable Longitudinal Methylation Levels at the CpG Sites Flanking the CTG Repeat of DMPK in Patients with Myotonic Dystrophy Type 1
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Genes
Volume 11
Issue 8
10.3390/genes11080937
genes-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer

by
Kun-Lung Li
1,*,
Keisuke Nakashima
1,
Jun Inoue
2 and
Noriyuki Satoh
1
1
Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate School, Onna, Okinawa 904–0495, Japan
2
Center for Earth Surface System Dynamics, Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba 277-8564, Japan
*
Author to whom correspondence should be addressed.
Genes 2020, 11(8), 937; https://doi.org/10.3390/genes11080937
Submission received: 23 June 2020 / Revised: 8 August 2020 / Accepted: 10 August 2020 / Published: 13 August 2020
(This article belongs to the Section Animal Genetics and Genomics)
Browse Figures
Versions Notes

Abstract

:
Horizontal gene transfer (HGT) is the movement of genetic material between different species. Although HGT is less frequent in eukaryotes than in bacteria, several instances of HGT have apparently shaped animal evolution. One well-known example is the tunicate cellulose synthase gene, CesA, in which a gene, probably transferred from bacteria, greatly impacted tunicate evolution. A Glycosyl Hydrolase Family 6 (GH6) hydrolase-like domain exists at the C-terminus of tunicate CesA, but not in cellulose synthases of other organisms. The recent discovery of another GH6 hydrolase-like gene (GH6-1) in tunicate genomes further raises the question of how tunicates acquired GH6. To examine the probable origin of these genes, we analyzed the phylogenetic relationship of GH6 proteins in tunicates and other organisms. Our analyses show that tunicate GH6s, the GH6-1 gene, and the GH6 part of the CesA gene, form two independent, monophyletic gene groups. We also compared their sequence signatures and exon splice sites. All tunicate species examined have shared splice sites in GH6-containing genes, implying ancient intron acquisitions. It is likely that the tunicate CesA and GH6-1 genes existed in the common ancestor of all extant tunicates.

1. Introduction

Horizontal gene transfer (HGT, or lateral gene transfer) is the movement of genetic material between unrelated organisms. Bacterial genomes are greatly shaped by HGT and some of them may contain more than 10% transferred genes [1,2,3]. Although animals usually inherit genetic information from parents [4], many horizontally transferred genes are maintained in animal genomes and expressed [5,6,7]. HGT may well be one of the most important forces shaping animal evolution [5]. One well-recognized example of HGT is the enzyme, tunicate cellulose synthase. Tunicates are the closest living relatives of vertebrates [8,9]. Among animals, tunicates have a unique ability to synthesize and utilize cellulose [10,11,12,13,14]. The tunicate cellulose synthase gene (CesA) has an unusual structure. It contains not only a glycosyltransferase (GT2) domain (Glycosyltransferase-like family 2, Pfam PF13641, or CESA_CelA_like, Conserved Domain Database cd06421), but also a Glycosyl Hydrolase family 6 domain (GH6, Pfam: PF01341) [10,11,12,13]. Notably, the GH6 domain of tunicate CesA protein (CesA-GH6) contains an amino acid substitution in the putative active site [10,15] and it may lack hydrolytic activity. A previous analysis of tunicate cellulose synthase failed to identify a cellulose synthase gene in the genome of any other animal [14]. The same analysis also revealed another independent gene (a group of possibly orthologous genes) in tunicate genomes, named GH6-1, which contains a GH6 domain [14].
Based on molecular phylogeny and the unique structure of tunicate CesA, Nakashima et al. [10] hypothesized that a bacterial genomic region that contained both a GT2/CesA gene and a GH6 gene, was transferred horizontally to ancestral tunicates, and that the two genes/domains later merged to form the tunicate CesA gene. This hypothesis was further strengthened, when it was observed that actinobacterial genomes contain GC-rich sequences that can be transformed into enhancers in the tunicate cellular environment [16]. Until now, GH6-domain-containing genes have been found in bacteria, fungi, tunicates, and a few other eukaryotes. Because of sequence divergence between tunicate CesA-GH6 domains and GH6-1 proteins, previous studies could not determine the relationship of tunicate CesA-GH6 and GH6-1 proteins with those of other organisms [10,12]. In other words, the evolutionary relationship of the tunicate GH6-1 gene with other GH6-containing genes remains uncertain.
In eukaryotes, conservation of splice sites (location of boundaries between exons and introns) is often found among orthologous genes [17,18]. Assuming that tunicate GH6-containing genes were transferred horizontally from bacteria, acquisition of spliceosomal introns in tunicate CesA-GH6 or GH6-1 genes could be interpreted as a eukaryote-specific character [19,20]. A previous survey [21] found that no splice sites were shared between the tunicate CesA genes and plant cellulose synthase genes; therefore, it was concluded that ancient CesA genes without introns transferred into tunicate genomes and plant genomes independently.
The foregoing finding raised the question of how the tunicate ancestor acquired the precursor of the CesA-GH6 and GH6-1 genes. Three possible evolutionary scenarios have been proposed (Figure 1) [10,14]. Scenario 1: Two GH6 genes were transferred, one of which merged with a GT2-containing gene from the same prokaryote genomic region transferred to an ancestral tunicate and formed the tunicate CesA gene. The second GH6-gene gave rise to the current GH6-1. Scenario 2: A GH6 gene was transferred and duplicated. After a single transfer of prokaryote GT2-GH6 region into a tunicate genome, a duplication occurred. One copy did not include or retain the GT2 part and became GH6-1, while the other copy (an ancient ‘GH6 gene’) merged with the GT2 domain and became part of tunicate CesA (joined GT2-GH6 domains). Scenario 3: A GT2-gene and a GH6-gene transferred independently into an ancestral tunicate. The GH6-gene duplicated thereafter. One copy of the GH6 gene fused with the GT2 gene to form the tunicate CesA gene. The other copy remained an independent GH6-1 gene.
In this study, we assessed possible origins of tunicate GH6s by examining phylogenetic relationships of GH6-containing genes in diverse organisms. We also compared sequence characters and exon boundaries among tunicate GH6 domains to understand their evolutionary changes in tunicate genomes.

2. Materials and Methods

2.1. Re-Analyzing Phylogenetic Relationships of GH6-Containing Proteins of Tunicates, Fungi, Other Eukaryotes, and Bacteria

We reanalyzed genes and gene models of tunicate cellulose synthase (CesA) and GH6-1, characterized in previous studies (Table 1) [10,11,12,14]. Corresponding gene models and genomic information were retrieved from: NCBI GenBank (reported genes and sequence assemblies: Salpa thompsoni genomic assemblies GCA_001749815.1 [22] and transcriptome GFCC00000000.1 [23], and the Ciona savignyi transcriptome GGEI00000000.1 [24]), the Ghost database for Ciona intestinalis type A (Kyoto University) [25,26,27], the Botryllus schlosseri Genome Project (transcripts only, Stanford University) [28,29], the OikoBase for Oikopleura dioica [30,31], and the Aniseed database (transcripts and genomes of all other species, as well as the genomes of C. savignyi and B. schlosseri) [32,33]. (Please note that the name of the species ‘Ciona intestinalis type A’ used here follows the name of archived sequence data in databases, including NCBI).
Although the recorded transcripts or annotated gene models were retrieved, we wished to examine whether there is any hidden GH6-encoding genetic information that failed to be annotated as a gene model in each tunicate genome. We first recorded the genomic location (the coordinates on chromosomes, scaffolds, or contigs) of each predicted GH6-1 and CesA gene. When the genomic locations of transcript/models were unknown, as in the cases of C. intestinalis type A, C. savignyi, S. thompsoni, and O. dioica, the GH6-containing transcripts were used to search (blastn in BLAST, Basic Local Alignment Search Tool, using default parameters) against its corresponding genome/genomic assembly: the databases used were listed as above. The genomic locations of tunicate GH6-containing genes were listed in Supplementary Table S3. Next, we used the GH6 domains in C. intestinalis type A predicted proteins of CesA (GenBank: BAD10864.1) and GH6-1 (NCBI: XP_002119579.1) as queries to search (tblastn in BLAST, with default parameters, e-value threshold = 1×10−10) against the other seven tunicates’ genomic database or assemblies and used O. dioica predicted proteins (GH6-1, CBY09680.1 and CesA2, BAJ65326.1) to search (tblastn, with default parameters, e-value threshold = 1 × 10−10) the C. intestinalis type A genome and recorded the genomic locations of results. We found that the location of retrieved transcripts/gene models mostly matched with the BLAST search (tblastn) results, with minor exceptions: a few additional open reading frames (ORF) or short gene models were newly discovered. For example, an ORF of M. oculata coding for a 39-amino-acid (AA) peptide and a gene model of B. schlosseri, Boschl.CG.Botznik2013.chr9.g44329, coding for an 166-AA peptide, were found in BLAST searches. These short peptides/gene models have similar sequences to a GH6 domain, but those are either far shorter (less than 140 AA) than a typical GH6 domain (Pfam PF01341, with sizes of around 300 AA) or were evaluated as ‘no significance’ in protein profile searches (hmmscan, HmmerWeb version 2.41.1, searched against the Pfam database [34,35]). Therefore, we interpreted that there is no better hidden representative of GH6 genes in these genomes.
We prepared an expanded sequence alignment including more bacterial/fungal GH6 sequences for the phylogenetic analysis. The same two C. intestinalis type A protein models (CesA, BAD10864.1 and GH6-1, XP_002119579.1) were used as queries to perform BLAST searches of the NCBI non-redundant protein (nr) database. The blastp (protein-protein BLAST) algorithm was selected, with default parameters (word size = 6; matrix = BLOSUM62; gap cost existence:11, extension:1; conditional compositional score matrix adjustment). A strategy was used to achieve broad sampling of GH6-containing proteins across different taxa. First, the query was used to search all nr sequences excluding tunicates, and the results with the lowest e-values were all sequences from the genus Streptomyces. A second search was carried out against “All data excluding tunicates and Streptomyces.” Several subsequent searches were performed stepwise, excluding higher taxa (Streptomycetales, Actinobacteria, or Bacteria). Another approach was to search only “Archaea”, “Fungi”, or “Eukaryotes, excluding tunicates and fungi.” A GH6 protein (NCBI: WP_094052291.1) from Streptomyces was also used as a query to expand the search results in several eukaryotic taxa (Table 2). However, two questionable ‘eukaryotic’ results, showing higher similarity to bacterial proteins and linkages to other probable bacterial genes, were excluded (Table 2). A few selected bacterial and fungal sequences that were used in a previous phylogenetic analysis [12] were also included in later analyses. In some results, long sequences included conserved domains other than GH6, which were confirmed using InterPro searches (online searches against all available databases) [36]. Those extra domains were excised before downstream analyses. All the selected sequences contained a GH6 domain (Pfam: PF01341), which was confirmed by a hmmscan examination (HmmerWeb version 2.41.1, searched against Pfam database) [34,35]; a GH6 domain in each sequence was identified with an Individual E-value smaller than 1×10−5. The multiple sequence alignments were built with MAFFT v7 online server (strategy: L-INS-I iterative refinement recommended for <200 sequences with one conserved domain and long gaps) [37,38]. Poorly aligned regions were removed using trimAl v1.2 [39] when more than 65% of the selected sequences showed gaps in a given position. The appropriate amino acid substitution model was selected using Prottest 3.4.2 (with default parameters) [40] before a maximum likelihood phylogenetic analysis. Phylogenetic reconstructions were performed with MrBayes 3.2.7a (nucmodel = protein, aamodelpr = mixed, ngen = 2,500,000, nchains = 1) [41] or RAxML-HPC Blackbox v8.2.12 (substitution model: PROTCATWAGF, rapid bootstrap with automatic bootstopping) [42] via CIPRES Science Gateway [43]. Consensus trees were visualized with FigTree [44].

2.2. Sequence Comparison

Signatures of GH6 proteins were compared with information on PROSITE [45]. Some genes or gene models in the databases had been annotated with exon boundaries. When exon information of genes or gene models was unknown, sequences of transcripts were used to search (blastn, with default parameters) against the corresponding genomic databases: the Ghost database [25,26,27] for C. intestinalis type A, NCBI genome assembly GCA_001749815.1 for S. thompsoni genomic assembly [22], OikoBase [30,31] for O. dioica, and the Aniseed database BLAST tool for other tunicate species [32,33]. Coding parts of transcripts and genomic sequences were then compared with the Splign utility (with default parameters) at NCBI [46]. Tunicate GH6-containing proteins were aligned with MAFFT v7 server (strategy: L-INS-I) [37,38] for splice site (exon-boundary) comparisons.

3. Results

3.1. Tunicate CesA-GH6 Domains and Tunicate GH6-1 Genes Represent Two Independent Monophyletic Groups

To determine whether GH6-1 genes represent a monophyletic group distinct from tunicate CesA genes and to understand the relationship of GH6-1 with GH6 proteins in other organisms, we used amino acid sequences of eight predicted tunicate CesA-GH6 domains, sequences of eight predicted GH6-1 proteins, and many predicted GH6 protein sequences from bacteria, fungi, and various eukaryotes (Supplementary Table S1) to reconstruct phylogenetic trees (Figure 2, Supplementary Figures S1 and S2). Both Bayesian inference (Figure 2A) and maximum likelihood (ML) (Figure 2B) approaches provided trees supporting a close relationship of tunicate CesA-GH6 and GH6-1. In addition, CesA-GH6 sequences and GH6-1 sequences formed two separate clusters, although the ML bootstrap support values were only 83% for the GH6-1 clade and 61% for the CesA-GH6 clade.

3.2. The Origin of Tunicate GH6 Domains Is Hard to Deduce

Nonetheless, our analyses failed to determine the relationship of tunicate sequences among other GH6 proteins. Although in these trees, tunicate sequences were clustered with many fungal GH6 proteins, some other eukaryotic GH6s (from red algae (Rhodophyta), Haptista, and the SAR supergroup), and a proteobacterial GH6 (YP_001618727.1, Sorangium cellulosum), the Bayesian posterior probability (Figure 2A) and ML bootstrap support (Figure 2B) for this clustering were low. Notably, branches leading to tunicate sequences were longer than branches to other sequence clusters.

3.3. Many Tunicate GH6-1 Proteins Maintain the Probable Active Site, in Contrast to Tunicate CesA Proteins

With the sequence alignment of tunicate GH6-1 and CesA proteins, we compared their sequence signatures to those of other GH6 proteins. The enzymatic activity of Exoglucanase 2 (Cel6A) of Hypocrea jecorina (formerly Trichoderma reesei) was well characterized [15]. The aspartic acid at position 221 of H. jecorina Cel6A (Hje-D221) serves as the catalytic center [15]. We found that in many tunicate GH6-1 proteins, an aspartic acid can be aligned to the catalytic H. jecorina D221 (Figure 3A), except for SthGH6-1b (E197) and OdiGH6-1 (K211). However, the catalytic aspartic acid was not conserved in tunicate CesA (Figure 3A). Tunicate proteins also show a sequence environment that almost matches (8–9 out of 10 amino acids) the conserved ‘signature 2’ of GH6 (Figure 3C: PROSITE PS00656: [LIVMYA]-[LIVA]-[LIVT]-[LIV]-E-P-D-[SAL]-[LI]-[PSAG]).
Another signature of GH6 that also contributes to catalytic ability is PS00655 (Figure 3B, including another important aspartic acid, D175, in the H. jecorina protein [15]), but this signature was not conserved in tunicate GH6-containing proteins. In the aligned region, ≤40% of amino acids matched the signature pattern.

3.4. Splice Site Conservation in Tunicate CesA Genes or GH6-1 Genes

We arranged the positions of coding exon splice sites (exon boundaries) of tunicate GH6-1 and the GH6 domain of CesA genes and then registered all sites to an aligned amino acid sequence matrix for comparison. For example, the splice site V217.frame+2 of CinGH6-1 means that the last nucleotide of an exon locates at the second codon position for amino acid 217 (valine) of C. intestinalis type A GH6-1 protein. Similarly, the site K316.frame+3 means that the last nucleotide of an exon is the nucleotide of the third codon position for amino acid 316 (lysine).
Several splice sites matched among tunicate GH6-1 proteins (Table 3), and these matching splice sites also have the same frame as the exon-intron boundary. Therefore, we consider them genuine shared splice sites. For example, the site Cin316 was shared by eight GH6-1 genes from seven tunicate species.
An obscure case was that of the O. dioica CesA1 R1100 site. Although one O. dioica exon boundary was located in a codon for an arginine that could be aligned to the amino acids of splice site Cin316 of GH6-1 proteins, the location of the splice site was shifted by one nucleotide. Our results do not indicate that O. dioica CesA1 shares this splice site with tunicate GH6-1 genes.
Excluding the foregoing case, we found no splice site shared between tunicate GH6-1 and CesA. However, several other splice sites are shared within CesA protein GH6 domains (Supplementary Table S2).

3.5. Genomic Locations of Tunicate CesA Genes and GH6-1 Genes are Separated

During the comparison of tunicate GH6-1 and CesA-GH6 transcripts to genomic DNA, we noted that most GH6-1 genes and CesA-GH6 genes are located separately in the genome (Supplementary Table S3). In C. intestinalis type A, for which a chromosome-level genome is available [47], the GH6-1 and CesA genes are located in chromosomes 3 and 7, respectively. Although gene models of a GH6-1 and a GH6-2 appear on chromosome 9 of Botryllus schlosseri, these two gene models are separated by about 2.65 million base pairs. In other species, although draft genomes are in scaffold-level assemblies, GH6-1 and CesA-GH6 do not appear on the same scaffold/contig. These results suggest a reduced likelihood of tandem duplication of an ancestral GH6 gene.

4. Discussion

4.1. Two GH6-Containing Genes Exist in Tunicate Genomes

In this study, we first tried to resolve the relationship of a recently discovered tunicate GH6-containing gene (GH6-1), the GH6 part of the tunicate CesA gene (called CesA-GH6), and GH6-containing genes from other organisms. The result was that tunicate CesA-GH6 and GH6-1 sequences form two clusters (Figure 2), indicating that these are two monophyletic groups and that both were inherited from the tunicate common ancestor. On the other hand, in phylogenetic reconstructions, the grouping of tunicate GH6-containing proteins and other GH6s was not conclusive (Figure 2). There were long branches that thwarted conclusive results regarding the relationship of tunicate GH6-containing proteins and those of other organisms. We suspect that the highly evolved tunicate GH6-containing-proteins cause long-branch attraction, adversely affecting tree topologies. Based on current phylogenetic trees, we could not confidently propose a non-tunicate GH6 protein(s) that represents the closest relative(s) to tunicate GH6-containing proteins. Considering branch lengths and the tree topology of GH6 proteins, it is possible that an ancient GH6 gene evolved highly, soon after it was transferred into an ancestral tunicate. After the transfer event, this GH6 gene likely duplicated in the tunicate genome. We drew this conclusion because of clustering of tunicate CesA-GH6 and GH6-1 groups, in which no genes of other organisms were inserted. Therefore, either scenario 2 or 3 in Figure 1 could explain the origin of tunicate GH6-containing genes. However, as we could not propose a candidate donor species/lineage of tunicate GH6s, we cannot directly evaluate the two possible scenarios further.
Assuming that tunicate GH6-genes were acquired via HGT event(s), no other tunicate genes would help to resolve the current, ambiguous tree topology. On the other hand, it is intriguing that many, but not all, GH6 proteins from other eukaryotes (including GH6s of fungi, the SAR supergroup, Haptista, and red algae) were clustered close to tunicate CesA-GH6. Recently, it was shown that some fungi retain many genes acquired from bacteria [48]. Therefore, future disclosures of eukaryotic genes similar to tunicate GH6 genes may provide important information on possible horizontal gene transfer events. As we found no GH6 genes in Archaea (Table 2), GH6 genes may have been transferred from bacteria to multiple eukaryotes in parallel. Alternatively, GH6 genes could also have been transferred between different eukaryotic organisms.
The separate genomic locations of tunicate CesA genes and GH6-1 genes (Supplementary Table S3) imply that the two genes did not stem from recent tandem duplication events, so these genes have been regulated in different genomic contexts.

4.2. Lineage-Specific Gene Content Change Along with Sequence Signature Conservation

We found multiple transcripts or gene models representing GH6-1 (or multiple CesA-GH6s) in the genomes of some tunicate species (Table 1 and Supplementary Table S3). Some of them may represent true lineage-specific duplications, as in the case of the two CesA genes of O. dioica [12]. For example, the two S. thompsoni GH6-1 proteins have only 35% identical amino acids when aligned and compared. They also showed long terminal branches in phylogenetic trees. In addition, although the current S. thompsoni genome had been assembled into sub-chromosome level scaffolds, these two GH6-1 genes corresponded to different genomic scaffolds. However, some gene models and open reading frames are highly similar to (around 90% amino acid identity) and shorter than another gene model in the same genome. For example, one GH6-1 protein model of B. schlosseri (BscGH6-1b, g61144, chromosome unassigned) showed 97.5% identity to BscGH6-1 (g9326, chromosome 9). These could be more recently duplicated genes. Alternatively, these may just be different alleles annotated separately due to imperfections of software-based genome assembly and may not represent a true species-specific duplication. Some gene models contain the GH6 part, but not the CesA/GT2 part of the tunicate CesA gene. Based on our knowledge that a typical, complete tunicate CesA gene contains a CesA/GT2 part and a GH6 part, it is possible that the CesA/GT2 part of a complete tunicate CesA gene was erroneously predicted as another gene model in the aforementioned cases, similar to a previous observation on a sea urchin genome [49] and several amphioxus gene models [50]. We also found that one CesA model of M. oculata (MocCesAa, Moocul.CG.ELv1_2. S71617.g04842.01.t) is obviously larger. It also encodes a rhodopsin-like G protein-coupled receptor domain (Interpro: IPR000276) at its upstream end. It would require further studies to confirm whether it is a true merged gene, a mistake in genome assembly and annotation, or a polycistronic operon, similar to those of O. dioica or C. intestinalis type A [51,52].
This analysis of GH6-1 and CesA-GH6 sequence signatures shows that, although both tunicate GH6-1 proteins and CesA-GH6 domains contain a region that almost matches the conserved GH6-signature 2 (PROSITE PS00656), the probable catalytic aspartic acid exists only in GH6-1 proteins and not in CesA-GH6. This aspartic acid is conserved in most non-tunicate GH6 proteins (56 out of 58 sequences compared in this study). Mutation of this possible catalytic site in CesA genes probably occurred very early in an ancestral tunicate before the branching of the larvacean (Appendicularia) clade. Despite the loss of the aspartic acid, the conservation of other amino acids at the signature site may imply that this domain acquired novel function in tunicates. Nevertheless, whether tunicate GH6-1 proteins or CesA-GH6 domains possess any catalytic activity remains to be determined.

4.3. Shared Splice Sites Indicate the Ancient History of Tunicate GH6 Genes

In this study, we found several shared splice sites among tunicate GH6-1 genes. We also extended the comparison of shared splice sites of CesA genes to other tunicate species. As previously reported [12], 17 splice sites in CesA genes of C. intestinalis type A and C. savignyi are still conserved after about 100 million years of independent evolution [53]. In addition, a splice site shared by CesA2 of O. dioica and O. longicauda, CesA of Halocynthia roretzi, Molgula tectiformis, and two Ciona species was interpreted as support for common ancestry of all tunicate CesA genes [13]. In this study, although we found no other sites shared between genes of O. dioica and other tunicates, we found that many shared splice sites are present among GH6-containing genes from three other major clades of tunicates (Thaliacea + Phlebobranchia + Stolidobranchia). It is reasonable to assume that many shared introns were acquired after the branching of larvaceans and before the subsequent divergence of major tunicate clades.
There was no well-supported splice site shared between GH6-1 and CesA-GH6. Assuming that only one GH6 gene was transferred horizontally into an ancestral tunicate genome, the lack of shared splice sites between GH6-1 and CesA-GH6 may indicate that the ancient GH6 gene had no introns when it was transferred into the tunicate genome. This supports a previous interpretation about the CesA transfer event [21].
The obscure O. dioica CesA1 splice site (R1100) differs by just one nucleotide from the Cin316 splice site of GH6-1 genes. It may simply have resulted from an independent intron acquisition event. Alternatively, this could represent a shared splice site that experienced a one-nucleotide intron shift [54], but this requires further investigation. Moreover, no other CesA genes we examined show a splice site here. If the GH6 part of the ancient CesA gene contained that intron, other CesA genes must have undergone intron loss. Therefore, it is not a parsimonious explanation.
The presence of two CesA genes in O. dioica raised another question of whether tunicate CesA was duplicated before larvaceans diverged [12] (see also Figure 2). The observation that Ciona CesA genes share a splice site with OdiCesA2, but not OdiCesA1, may favor the scenario of early duplication [12]. In our analysis, the splice site discussed previously, Cin976, was found in the OdiCesA2 and CesA genes of at least six other tunicate species (Supplementary Table S2), but this splice site was not found in M. oculata. Therefore, it is possible that O. dioica had a lineage-specific duplication of the CesA gene and that one copy (CesA1) lost this intron.

4.4. Future Perspective

It is likely that a GH6-containing gene was transferred to and duplicated in ancient tunicate genomes before major tunicate lineages diverged. The two tunicate GH6-containing genes acquired different introns and have preserved part of that sequence signature. We anticipate that future larvacean transcriptomic studies that are complementary to recent larvacean genome projects (for example, [55]) will provide a better understanding of tunicate GH6-containing genes and tunicate genome evolution.
In plants, activity of cellulase is required to regulate cellulose synthesis and growth of cell walls [56]. Therefore, it is important to examine whether any hydrolase activity of tunicate GH6-containing proteins could also influence cellulose synthesis and physiology. One approach would be to examine enzymatic activity in vitro, and another would be to genetically manipulate animals using genome-editing methods [57,58]. These are subjects for future studies.

Supplementary Materials

The following are available online at https://www.mdpi.com/2073-4425/11/8/937/s1, Supplementary Table S1. Other GH6-containing proteins used in phylogenetic analyses. Supplementary Table S2. Shared splice sites in the GH6 domain of tunicate CesA proteins. Supplementary Table S3. Tunicate GH6-containing genes or gene models and their genomic locations. Supplementary Figure S1: The fully expanded phylogenetic tree of Figure 2A, showing Bayesian inference of phylogenetic relationships of GH6 proteins. Supplementary Figure S2: The fully expanded phylogenetic tree of Figure 2B, showing a maximum likelihood reconstruction of phylogenetic relationships of GH6 proteins.

Author Contributions

Conceptualization, K.-L.L. and N.S.; investigation and analysis, K.-L.L.; review and methodology, K.N. and J.I.; writing—original draft preparation, K.-L.L.; manuscript review and editing, K.N., J.I., and N.S.; supervision, N.S. All authors have read and agreed to the final version of manuscript.

Funding

This research was funded by an internal grant from the Okinawa Institute of Science and Technology Graduate School to Marine Genomics Unit.

Acknowledgments

We thank Steven Aird for editing the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Garcia-Vallve, S.; Romeu, A.; Palau, J. Horizontal gene transfer in bacterial and archaeal complete genomes. Genome Res. 2000, 10, 1719–1725. [Google Scholar] [CrossRef] [PubMed]
  2. Koonin, E.V.; Makarova, K.S.; Aravind, L. Horizontal gene transfer in prokaryotes: Quantification and classification. Annu. Rev. Microbiol. 2001, 55, 709–742. [Google Scholar] [CrossRef] [PubMed]
  3. Soucy, S.M.; Huang, J.; Gogarten, J.P. Horizontal gene transfer: Building the web of life. Nat. Rev. Genet. 2015, 16, 472–482. [Google Scholar] [CrossRef] [PubMed]
  4. Martin, W.F. Too much eukaryote LGT. Bioessays 2017, 39. [Google Scholar] [CrossRef]
  5. Boto, L. Horizontal gene transfer in the acquisition of novel traits by metazoans. Proc. Biol. Sci. 2014, 281, 20132450. [Google Scholar] [CrossRef] [Green Version]
  6. Dunning Hotopp, J.C. Horizontal gene transfer between bacteria and animals. Trends Genet. 2011, 27, 157–163. [Google Scholar] [CrossRef] [Green Version]
  7. Husnik, F.; McCutcheon, J.P. Functional horizontal gene transfer from bacteria to eukaryotes. Nat. Rev. Microbiol. 2018, 16, 67–79. [Google Scholar] [CrossRef]
  8. Delsuc, F.; Tsagkogeorga, G.; Lartillot, N.; Philippe, H. Additional molecular support for the new chordate phylogeny. Genesis 2008, 46, 592–604. [Google Scholar] [CrossRef] [Green Version]
  9. Putnam, N.H.; Butts, T.; Ferrier, D.E.; Furlong, R.F.; Hellsten, U.; Kawashima, T.; Robinson-Rechavi, M.; Shoguchi, E.; Terry, A.; Yu, J.K.; et al. The amphioxus genome and the evolution of the chordate karyotype. Nature 2008, 453, 1064–1071. [Google Scholar] [CrossRef] [Green Version]
  10. Nakashima, K.; Yamada, L.; Satou, Y.; Azuma, J.; Satoh, N. The evolutionary origin of animal cellulose synthase. Dev. Genes Evol. 2004, 214, 81–88. [Google Scholar] [CrossRef]
  11. Matthysse, A.G.; Deschet, K.; Williams, M.; Marry, M.; White, A.R.; Smith, W.C. A functional cellulose synthase from ascidian epidermis. Proc. Natl. Acad. Sci. USA 2004, 101, 986–991. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Sagane, Y.; Zech, K.; Bouquet, J.M.; Schmid, M.; Bal, U.; Thompson, E.M. Functional specialization of cellulose synthase genes of prokaryotic origin in chordate larvaceans. Development 2010, 137, 1483–1492. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Nakashima, K.; Nishino, A.; Horikawa, Y.; Hirose, E.; Sugiyama, J.; Satoh, N. The crystalline phase of cellulose changes under developmental control in a marine chordate. Cell Mol. Life Sci. 2011, 68, 1623–1631. [Google Scholar] [CrossRef] [PubMed]
  14. Inoue, J.; Nakashima, K.; Satoh, N. Orthoscope analysis reveals the presence of the cellulose synthase gene in all tunicate genomes but not in other animal genomes. Genes 2019, 10, 294. [Google Scholar] [CrossRef] [Green Version]
  15. Koivula, A.; Ruohonen, L.; Wohlfahrt, G.; Reinikainen, T.; Teeri, T.T.; Piens, K.; Claeyssens, M.; Weber, M.; Vasella, A.; Becker, D.; et al. The active site of cellobiohydrolase Cel6A from Trichoderma reesei: The roles of aspartic acids D221 and D175. J. Am. Chem. Soc. 2002, 124, 10015–10024. [Google Scholar] [CrossRef]
  16. Sasakura, Y.; Ogura, Y.; Treen, N.; Yokomori, R.; Park, S.J.; Nakai, K.; Saiga, H.; Sakuma, T.; Yamamoto, T.; Fujiwara, S.; et al. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate. Proc. Biol. Sci. 2016, 283. [Google Scholar] [CrossRef] [Green Version]
  17. Rogozin, I.B.; Wolf, Y.I.; Sorokin, A.V.; Mirkin, B.G.; Koonin, E.V. Remarkable interkingdom conservation of intron positions and massive, lineage-specific intron loss and gain in eukaryotic evolution. Curr. Biol. 2003, 13, 1512–1517. [Google Scholar] [CrossRef] [Green Version]
  18. Putnam, N.H.; Srivastava, M.; Hellsten, U.; Dirks, B.; Chapman, J.; Salamov, A.; Terry, A.; Shapiro, H.; Lindquist, E.; Kapitonov, V.V.; et al. Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization. Science 2007, 317, 86–94. [Google Scholar] [CrossRef] [Green Version]
  19. Patel, A.A.; Steitz, J.A. Splicing double: Insights from the second spliceosome. Nat. Rev. Mol. Cell Biol. 2003, 4, 960–970. [Google Scholar] [CrossRef]
  20. Nixon, J.E.; Wang, A.; Morrison, H.G.; McArthur, A.G.; Sogin, M.L.; Loftus, B.J.; Samuelson, J. A spliceosomal intron in Giardia lamblia. Proc. Natl. Acad. Sci. USA 2002, 99, 3701–3705. [Google Scholar] [CrossRef] [Green Version]
  21. Bhattachan, P.; Dong, B. Origin and evolutionary implications of introns from analysis of cellulose synthase gene. J. Syst. Evol. 2017, 55, 142–148. [Google Scholar] [CrossRef]
  22. Jue, N.K.; Batta-Lona, P.G.; Trusiak, S.; Obergfell, C.; Bucklin, A.; O’Neill, M.J.; O’Neill, R.J. Rapid evolutionary rates and unique genomic signatures discovered in the first reference genome for the Southern Ocean salp, Salpa thompsoni (Urochordata, Thaliacea). Genome Biol. Evol. 2016, 8, 3171–3186. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Batta-Lona, P.G.; Maas, A.E.; O’Neill, R.J.; Wiebe, P.H.; Bucklin, A. Transcriptomic profiles of spring and summer populations of the Southern Ocean salp, Salpa thompsoni, in the Western Antarctic Peninsula region. Polar Biol. 2017, 40, 1261–1276. [Google Scholar] [CrossRef]
  24. Wei, J.; Dong, B. Identification and expression analysis of long noncoding RNAs in embryogenesis and larval metamorphosis of Ciona savignyi. Mar. Genom. 2018, 40, 64–72. [Google Scholar] [CrossRef] [PubMed]
  25. Satou, Y.; Mineta, K.; Ogasawara, M.; Sasakura, Y.; Shoguchi, E.; Ueno, K.; Yamada, L.; Matsumoto, J.; Wasserscheid, J.; Dewar, K.; et al. Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: New insight into intron and operon populations. Genome Biol. 2008, 9, R152. [Google Scholar] [CrossRef] [Green Version]
  26. Satou, Y.; Kawashima, T.; Shoguchi, E.; Nakayama, A.; Satoh, N. An integrated database of the ascidian, Ciona intestinalis: Towards functional genomics. Zool. Sci. 2005, 22, 837–843. [Google Scholar] [CrossRef] [Green Version]
  27. Ghost Database. Available online: http://ghost.zool.kyoto-u.ac.jp/download_kh.html (accessed on 17 December 2019).
  28. Voskoboynik, A.; Neff, N.F.; Sahoo, D.; Newman, A.M.; Pushkarev, D.; Koh, W.; Passarelli, B.; Fan, H.C.; Mantalas, G.L.; Palmeri, K.J.; et al. The genome sequence of the colonial chordate, Botryllus schlosseri. eLife 2013, 2, e00569. [Google Scholar] [CrossRef]
  29. Botryllus schlosseri Genome Project. Available online: http://botryllus.stanford.edu/botryllusgenome/ (accessed on 28 February 2020).
  30. Danks, G.; Campsteijn, C.; Parida, M.; Butcher, S.; Doddapaneni, H.; Fu, B.; Petrin, R.; Metpally, R.; Lenhard, B.; Wincker, P.; et al. OikoBase: A genomics and developmental transcriptomics resource for the urochordate Oikopleura dioica. Nucleic Acids Res. 2013, 41, D845–D853. [Google Scholar] [CrossRef]
  31. OikoBase. Available online: http://oikoarrays.biology.uiowa.edu/Oiko/index.html (accessed on 25 December 2019).
  32. Tassy, O.; Dauga, D.; Daian, F.; Sobral, D.; Robin, F.; Khoueiry, P.; Salgado, D.; Fox, V.; Caillol, D.; Schiappa, R.; et al. The ANISEED database: Digital representation, formalization, and elucidation of a chordate developmental program. Genome Res. 2010, 20, 1459–1468. [Google Scholar] [CrossRef] [Green Version]
  33. Aniseed. Available online: https://www.aniseed.cnrs.fr/ (accessed on 20 December 2019).
  34. Potter, S.C.; Luciani, A.; Eddy, S.R.; Park, Y.; Lopez, R.; Finn, R.D. HMMER web server: 2018 update. Nucleic Acids Res. 2018, 46, W200–W204. [Google Scholar] [CrossRef] [Green Version]
  35. hmmscan. Available online: https://www.ebi.ac.uk/Tools/hmmer/search/hmmscan (accessed on 6 August 2020).
  36. InterPro. Available online: https://www.ebi.ac.uk/interpro/ (accessed on 1 March 2020).
  37. Katoh, K.; Standley, D.M. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Mol. Biol. Evol. 2013, 30, 772–780. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Katoh, K.; Rozewicki, J.; Yamada, K.D. MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform. 2019, 20, 1160–1166. [Google Scholar] [CrossRef] [Green Version]
  39. Capella-Gutierrez, S.; Silla-Martinez, J.M.; Gabaldon, T. trimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 2009, 25, 1972–1973. [Google Scholar] [CrossRef] [PubMed]
  40. Darriba, D.; Taboada, G.L.; Doallo, R.; Posada, D. ProtTest 3: Fast selection of best-fit models of protein evolution. Bioinformatics 2011, 27, 1164–1165. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  41. Ronquist, F.; Teslenko, M.; van der Mark, P.; Ayres, D.L.; Darling, A.; Hohna, S.; Larget, B.; Liu, L.; Suchard, M.A.; Huelsenbeck, J.P. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 2012, 61, 539–542. [Google Scholar] [CrossRef] [Green Version]
  42. Stamatakis, A. RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014, 30, 1312–1313. [Google Scholar] [CrossRef]
  43. Miller, M.A.; Pfeiffer, W.; Schwartz, T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In Proceedings of Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, USA, 14–14 November 2010; pp. 1–8. [Google Scholar]
  44. Rambaut, A. FigTree v1.4. Available online: http://tree.bio.ed.ac.uk/software/figtree/ (accessed on 26 November 2018).
  45. Sigrist, C.J.; de Castro, E.; Cerutti, L.; Cuche, B.A.; Hulo, N.; Bridge, A.; Bougueleret, L.; Xenarios, I. New and continuing developments at PROSITE. Nucleic Acids Res. 2012, 41, D344–D347. [Google Scholar] [CrossRef] [Green Version]
  46. Kapustin, Y.; Souvorov, A.; Tatusova, T.; Lipman, D. Splign: Algorithms for computing spliced alignments with identification of paralogs. Biol. Direct 2008, 3, 20. [Google Scholar] [CrossRef] [Green Version]
  47. Satou, Y.; Nakamura, R.; Yu, D.; Yoshida, R.; Hamada, M.; Fujie, M.; Hisata, K.; Takeda, H.; Satoh, N. A nearly complete genome of Ciona intestinalis type A (C. robusta) reveals the contribution of inversion to chromosomal evolution in the genus Ciona. Genome Biol. Evol. 2019, 11, 3144–3157. [Google Scholar] [CrossRef] [Green Version]
  48. Murphy, C.L.; Youssef, N.H.; Hanafy, R.A.; Couger, M.B.; Stajich, J.E.; Wang, Y.; Baker, K.; Dagar, S.S.; Griffith, G.W.; Farag, I.F.; et al. Horizontal gene transfer as an indispensable driver for evolution of Neocallimastigomycota into a distinct gut-dwelling fungal lineage. Appl. Environ. Microbiol. 2019, 85. [Google Scholar] [CrossRef] [Green Version]
  49. Tu, Q.; Cameron, R.A.; Worley, K.C.; Gibbs, R.A.; Davidson, E.H. Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis. Genome Res. 2012, 22, 2079–2087. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  50. Li, K.L.; Lu, T.M.; Yu, J.K. Genome-wide survey and expression analysis of the bHLH-PAS genes in the amphioxus Branchiostoma floridae reveal both conserved and diverged expression patterns between cephalochordates and vertebrates. Evodevo 2014, 5, 20. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  51. Ganot, P.; Kallesoe, T.; Reinhardt, R.; Chourrout, D.; Thompson, E.M. Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome. Mol. Cell Biol. 2004, 24, 7795–7805. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Satou, Y.; Hamaguchi, M.; Takeuchi, K.; Hastings, K.E.; Satoh, N. Genomic overview of mRNA 5′-leader trans-splicing in the ascidian Ciona intestinalis. Nucleic Acids Res. 2006, 34, 3378–3388. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Delsuc, F.; Philippe, H.; Tsagkogeorga, G.; Simion, P.; Tilak, M.K.; Turon, X.; Lopez-Legentil, S.; Piette, J.; Lemaire, P.; Douzery, E.J.P. A phylogenomic framework and timescale for comparative studies of tunicates. BMC Biol. 2018, 16, 39. [Google Scholar] [CrossRef] [Green Version]
  54. Fekete, E.; Flipphi, M.; Ag, N.; Kavalecz, N.; Cerqueira, G.; Scazzocchio, C.; Karaffa, L. A mechanism for a single nucleotide intron shift. Nucleic Acids Res. 2017, 45, 9085–9092. [Google Scholar] [CrossRef] [Green Version]
  55. Naville, M.; Henriet, S.; Warren, I.; Sumic, S.; Reeve, M.; Volff, J.N.; Chourrout, D. Massive changes of genome size driven by expansions of non-autonomous transposable elements. Curr. Biol. 2019, 29, 1161–1168. [Google Scholar] [CrossRef] [Green Version]
  56. Vain, T.; Crowell, E.F.; Timpano, H.; Biot, E.; Desprez, T.; Mansoori, N.; Trindade, L.M.; Pagant, S.; Robert, S.; Hofte, H.; et al. The cellulase KORRIGAN is part of the cellulose synthase complex. Plant Physiol. 2014, 165, 1521–1532. [Google Scholar] [CrossRef] [Green Version]
  57. Treen, N.; Yoshida, K.; Sakuma, T.; Sasaki, H.; Kawai, N.; Yamamoto, T.; Sasakura, Y. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona. Development 2014, 141, 481–487. [Google Scholar] [CrossRef] [Green Version]
  58. Sasaki, H.; Yoshida, K.; Hozumi, A.; Sasakura, Y. CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 2014, 56, 499–510. [Google Scholar] [CrossRef] [Green Version]
Genes 11 00937 g001 550
Figure 1. Hypotheses on the origin of tunicate GH6 domain-containing genes. Three scenarios have been proposed to explain the existence of two GH6 domain-containing genes in extant tunicate genomes.
Figure 1. Hypotheses on the origin of tunicate GH6 domain-containing genes. Three scenarios have been proposed to explain the existence of two GH6 domain-containing genes in extant tunicate genomes.
Genes 11 00937 g001
Genes 11 00937 g002 550
Figure 2. Phylogenetic trees of GH6-containing proteins constructed by Bayesian inference (A) and maximum likelihood (B). All tunicate sequences formed a cluster. The cluster was further divided into two subclusters of CesA-GH6 domains and GH6-1 proteins. However, the clustering of tunicate GH6 sequences with GH6 proteins of other organisms was not well-supported. Rooting was arbitrary in both panels. Numbers next to internal nodes or branches represent posterior probabilities (in panel A) or bootstrap support (in panel B) of the neighboring branch. The same trimmed multiple sequence alignment was used as input for both analyses. Bayesian inference was performed with MrBayes using a mixed substitution model (aamodelpr = mixed). The analysis was terminated after 2,500,000 generations as the standard deviation of split frequencies remained as a stable 0.126917 after generation 1,830,000, although this analysis could not reach an ideal convergence due to short sequence lengths and divergent data. The maximum likelihood analysis was performed with RAxML-HPC BlackBox on CIPRES Science Gateway. The WAG amino acid substitution model with empirical base frequencies was selected and bootstrapping was automatically stopped after 804 cycles. The starting part of sequence names represents its source organism category, in alphabetical order: a, Actinobacteria, excluding Streptomyces; b, Bacteria excluding Actinobacteria; f, fungi; s, genus Streptomyces; T, tunicates. Fully-expanded trees are shown as supplementary figures. Scales represent expected changes per site.
Figure 2. Phylogenetic trees of GH6-containing proteins constructed by Bayesian inference (A) and maximum likelihood (B). All tunicate sequences formed a cluster. The cluster was further divided into two subclusters of CesA-GH6 domains and GH6-1 proteins. However, the clustering of tunicate GH6 sequences with GH6 proteins of other organisms was not well-supported. Rooting was arbitrary in both panels. Numbers next to internal nodes or branches represent posterior probabilities (in panel A) or bootstrap support (in panel B) of the neighboring branch. The same trimmed multiple sequence alignment was used as input for both analyses. Bayesian inference was performed with MrBayes using a mixed substitution model (aamodelpr = mixed). The analysis was terminated after 2,500,000 generations as the standard deviation of split frequencies remained as a stable 0.126917 after generation 1,830,000, although this analysis could not reach an ideal convergence due to short sequence lengths and divergent data. The maximum likelihood analysis was performed with RAxML-HPC BlackBox on CIPRES Science Gateway. The WAG amino acid substitution model with empirical base frequencies was selected and bootstrapping was automatically stopped after 804 cycles. The starting part of sequence names represents its source organism category, in alphabetical order: a, Actinobacteria, excluding Streptomyces; b, Bacteria excluding Actinobacteria; f, fungi; s, genus Streptomyces; T, tunicates. Fully-expanded trees are shown as supplementary figures. Scales represent expected changes per site.
Genes 11 00937 g002
Genes 11 00937 g003 550
Figure 3. Amino acid conservation of tunicate GH6-domain-containing proteins. (A) GH6-1 proteins from ascidians and the GH6-1a from Salpa thompsoni have aspartic acids that correspond to the catalytic center of fungal Cel6A protein; however, another S. thompsoni GH6-1 protein (SthGH6-1b), an Oikopleura GH6-1 protein, and tunicate CesA proteins show other amino acids at this site. Similar amino acids under the BLOSUM62 matrix are color-shaded. HjeCel6A: H. jecorina Exoglucanase 2, UniProtKB P07987.1. (B–C) Sequence logos of Glycosyl Hydrolases Family 6 Signature 1 (PROSITE entry PS00655, panel B) and Glycosyl Hydrolases Family 6 Signature 2 (PROSITE entry PS00656, panel C), showing the amino acid frequency of each site.
Figure 3. Amino acid conservation of tunicate GH6-domain-containing proteins. (A) GH6-1 proteins from ascidians and the GH6-1a from Salpa thompsoni have aspartic acids that correspond to the catalytic center of fungal Cel6A protein; however, another S. thompsoni GH6-1 protein (SthGH6-1b), an Oikopleura GH6-1 protein, and tunicate CesA proteins show other amino acids at this site. Similar amino acids under the BLOSUM62 matrix are color-shaded. HjeCel6A: H. jecorina Exoglucanase 2, UniProtKB P07987.1. (B–C) Sequence logos of Glycosyl Hydrolases Family 6 Signature 1 (PROSITE entry PS00655, panel B) and Glycosyl Hydrolases Family 6 Signature 2 (PROSITE entry PS00656, panel C), showing the amino acid frequency of each site.
Genes 11 00937 g003
Table
Table 1. Tunicate GH6-containing genes or gene models and related genes analyzed in this study.
Table 1. Tunicate GH6-containing genes or gene models and related genes analyzed in this study.
SpeciesDomain ContentShort Name of the Gene Used in this Manuscript *Source DatabaseAccession/ID of Gene, Transcript, or Protein Note
Ciona intestinalis type A (C. robusta)GH6CinGH6-1GenBankXM_002119543.4/XP_002119579.1
CesA+GH6CinCesAGenBankNM_001047983.1/BAD10864.1As reported in [10]
Ciona savignyiGH6CsaGH6-1GenBank (Transcriptome)GGEI01013363.1
CesA+GH6CsaCesAGenBankAY504665.1/AAR89623.1As reported in [11]
Salpa thompsoniGH6SthGH6-1aGenBank (Transcriptome)GFCC01117283.1Possible lineage-specific duplication
GH6SthGH6-1bGenBank (Transcriptome)GFCC01119318.1No possible catalytic Asp; possible lineage-specific duplication.
CesA+GH6SthCesAGenBank (Transcriptome)GFCC01072613.1
Molgula occidentalisGH6MoxGH6-1Aniseed databaseMoocci.CG.ELv1_2.S285391.g07021.01.t
CesA+GH6MoxCesAaAniseed databaseMoocci.CG.ELv1_2.S469068.g15915.01.tShort GH6 part
GH6(MoxCesAbGH6)Aniseed databaseMoocci.CG.ELv1_2.S469068.g15914.01.tVery short
GH6MoxCesAcGH6Aniseed databaseMoocci.CG.ELv1_2.S469068.g15913.01.t
Molgula oculataGH6MocGH6-1Aniseed databaseMoocul.CG.ELv1_2.S112948.g12660.01.t
CesA+GH6MocCesAaAniseed databaseMoocul.CG.ELv1_2.S71617.g04842.01.tRhodopsin-like GPCR domain at upstream part
GH6MocCesAbGH6Aniseed databaseMoocul.CG.ELv1_2.S69739.g04625.01.t
Botryllus schlosseriGH6BscGH6-1Botryllus schlosseri Genome Projectg9326
GH6(BscGH6-1b)Botryllus schlosseri Genome Projectg61144Short, similar to BscGH6-1
GH6BscCesAaGH6Botryllus schlosseri Genome Projectg44331Similar to BscCesAbGH6 (89.6% identity in the matching 222 AA region)
GH6BscCesAbGH6Botryllus schlosseri Genome Projectg45080Similar to BscCesAaGH6
Botrylloides leachiiGH6BleGH6-1Aniseed databaseBoleac.CG.SB_v3.S133.g02304.01.t
CesA+GH6BleCesAAniseed databaseBoleac.CG.SB_v3.S157.g03251.01.t
Oikopleura dioicaGH6OdiGH6-1OikoBase/GenBankGSOIDT00010490001/CBY09680.1
GH6(OdiGH6-1b)OikoBase/GenBankGSOIDT00021901001/CBY33927.198% identical to OdiGH6
CesA+GH6OdiCesA2GenBankAB543593.1/BAJ65326.1As reported in [12,13]
CesA+GH6OdiCesA1GenBankAB543594.1/BAJ65327.1As reported in [12,13]
* Gene names were assigned after considering phylogenetic information examined in this study and in that by Inoue et al. [14].
Table
Table 2. GH6 proteins in different taxa.
Table 2. GH6 proteins in different taxa.
TaxaGH6 presence?
BacteriaPresent
ArchaeaNot yet observed
EukaryotaOpisthokontaMetazoatunicatesPresent
Metazoa, except tunicateNo? Contamination? *1
FungiPresent
Opisthokonta, except Metazoa and fungiNot yet observed
ViridiplantaeNo? Contamination? *2
SAR-StramenopilesPresent
SAR-AlveolatePresent
SAR-RhizariaNot yet observed
HaptistaPresent
RhodophytaPresent
Other eukaryotesNot yet observed
*1: A GH6 protein in the Lucilia cuprina (a dipteran) genome project, XP_023300643.1, was very similar to bacterial GH6 proteins. It was located at a genomic scaffold that contained other probable bacterial genes. *2: A GH6 protein found in the Gossypium hirsutum (upland cotton) genome project, XP_016733546.1, was highly similar to bacterial GH6 proteins and it was located at a genomic scaffold that contained other probable bacterial genes. The above two cases were the only results that contained GH6 domains in each search. We treated these two cases as bacterial contaminants.
Table
Table 3. Splice site matches of tunicate GH6-1 proteins.
Table 3. Splice site matches of tunicate GH6-1 proteins.
Splice site name
Cin217Cin256Cin316
ProteinIntrons within coding regionSplice site residue & frame
CinGH6-13V217, +2G256, +1K316, +3
CsaGH6-13V223, +2G262, +1K322, +3
SthGH6-1a6E229, +2G268, +1P328, +3
SthGH6-1b5K230, +2G269, +1K329, +3
MoxGH6-13R222, +2G260, +1A320, +3
MocGH6-12n.s.*1 (R222)G260, +1A320, +3
BscGH6-15K335, +2G373, +1A433, +3
BleGH6-14K229, +2n.s.*2 (G285)A345, +3
OdiGH6-16n.s.*1 (N244)n.s.*2 (G282)n.s.*1 (K343)
OdiCesA1*38n.s.*1 (R1001)n.s.*2 (G1040)R1100*3, frame +2
All matching splice sites found in this study are C-terminal to the possible catalytic center: positions 178–187 in C. intestinalis type A GH6-1. *1: No splice (n.s.) site at the aligned amino acid and the amino acid is not conserved; *2: No splice (n.s.) site at the aligned amino acid, although this position encodes a conserved glycine; *3: The splice site OdiCesA1-R1100 could be aligned with splice site Cin316 of GH6-1 proteins at the amino acid level, but there is a one-nucleotide position difference and it may not represent a shared splice site.

Share and Cite

MDPI and ACS Style

Li, K.-L.; Nakashima, K.; Inoue, J.; Satoh, N. Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer. Genes 2020, 11, 937. https://doi.org/10.3390/genes11080937

AMA Style

Li K-L, Nakashima K, Inoue J, Satoh N. Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer. Genes. 2020; 11(8):937. https://doi.org/10.3390/genes11080937

Chicago/Turabian Style

Li, Kun-Lung, Keisuke Nakashima, Jun Inoue, and Noriyuki Satoh. 2020. "Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer" Genes 11, no. 8: 937. https://doi.org/10.3390/genes11080937

APA Style

Li, K.-L., Nakashima, K., Inoue, J., & Satoh, N. (2020). Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer. Genes, 11(8), 937. https://doi.org/10.3390/genes11080937

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop