Next Article in Journal
Chloroplast Genome Variation and Evolutionary Analysis of Olea europaea L.
Next Article in Special Issue
Splicing Characteristics of Dystrophin Pseudoexons and Identification of a Novel Pathogenic Intronic Variant in the DMD Gene
Previous Article in Journal
The Influences of Bioinformatics Tools and Reference Databases in Analyzing the Human Oral Microbial Community
Previous Article in Special Issue
RNA-Seq Analysis Reveals Localization-Associated Alternative Splicing across 13 Cell Lines
Article

Splicing Factor Transcript Abundance in Saliva as a Diagnostic Tool for Breast Cancer

1
Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel–Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel
2
Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem and Hadassah Medical Center, Jerusalem 9112102, Israel
3
Sharett Institute of Oncology, Hadassah-Hebrew University Medical Center, Hebrew University Medical School, Jerusalem 9112102, Israel
4
Department of Computer Science, Colorado State University, Fort Collins, CO 80523, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(8), 880; https://doi.org/10.3390/genes11080880
Received: 8 July 2020 / Revised: 27 July 2020 / Accepted: 30 July 2020 / Published: 3 August 2020
(This article belongs to the Special Issue Splicing: The New Frontier in Therapeutics)
Breast cancer is the second leading cause of death in women above 60 years in the US. Screening mammography is recommended for women above 50 years; however, 22% of breast cancer cases are diagnosed in women below this age. We set out to develop a test based on the detection of cell-free RNA from saliva. To this end, we sequenced RNA from a pool of ten women. The 1254 transcripts identified were enriched for genes with an annotation of alternative pre-mRNA splicing. Pre-mRNA splicing is a tightly regulated process and its misregulation in cancer cells promotes the formation of cancer-driving isoforms. For these reasons, we chose to focus on splicing factors as biomarkers for the early detection of breast cancer. We found that the level of the splicing factors is unique to each woman and consistent in the same woman at different time points. Next, we extracted RNA from 36 healthy subjects and 31 breast cancer patients. Recording the mRNA level of seven splicing factors in these samples demonstrated that the combination of all these factors is different in the two groups (p value = 0.005). Our results demonstrate a differential abundance of splicing factor mRNA in the saliva of breast cancer patients. View Full-Text
Keywords: splicing factors; saliva; diagnosis; breast cancer splicing factors; saliva; diagnosis; breast cancer
Show Figures

Figure 1

MDPI and ACS Style

Bentata, M.; Morgenstern, G.; Nevo, Y.; Kay, G.; Granit Mizrahi, A.; Temper, M.; Maimon, O.; Monas, L.; Basheer, R.; Ben-Hur, A.; Peretz, T.; Salton, M. Splicing Factor Transcript Abundance in Saliva as a Diagnostic Tool for Breast Cancer. Genes 2020, 11, 880. https://doi.org/10.3390/genes11080880

AMA Style

Bentata M, Morgenstern G, Nevo Y, Kay G, Granit Mizrahi A, Temper M, Maimon O, Monas L, Basheer R, Ben-Hur A, Peretz T, Salton M. Splicing Factor Transcript Abundance in Saliva as a Diagnostic Tool for Breast Cancer. Genes. 2020; 11(8):880. https://doi.org/10.3390/genes11080880

Chicago/Turabian Style

Bentata, Mercedes, Guy Morgenstern, Yuval Nevo, Gillian Kay, Avital Granit Mizrahi, Mark Temper, Ofra Maimon, Liza Monas, Reham Basheer, Asa Ben-Hur, Tamar Peretz, and Maayan Salton. 2020. "Splicing Factor Transcript Abundance in Saliva as a Diagnostic Tool for Breast Cancer" Genes 11, no. 8: 880. https://doi.org/10.3390/genes11080880

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop