Next Article in Journal
Cardiac Involvement in Dystrophin-Deficient Females: Current Understanding and Implications for the Treatment of Dystrophinopathies
Previous Article in Journal
Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target
Open AccessArticle

Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis

Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 111211, Colombia
Departamento de Salud Pública, Universidad Nacional de Colombia, Bogotá 111321, Colombia
Grupo de Investigaciones Biológicas de la Orinoquia, Unitrópico, Yopal 8500, Colombia
Hospital Local Santa María de Mompox, Programas Especiales (Lepra y TB), Mompox, Bolívar 132560, Colombia
Author to whom correspondence should be addressed.
Genes 2020, 11(7), 764;
Received: 14 June 2020 / Revised: 27 June 2020 / Accepted: 28 June 2020 / Published: 8 July 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Giardia intestinalis is an intestinal protozoan most commonly found in humans. It has been grouped into 8 assemblages (A-H). Markers such as the glutamate dehydrogenase gene, triose phosphate isomerase and beta-giardin (β-giardin) have been widely used for genotyping. In addition, different genetic targets have been proposed as a valuable alternative to assess diversity and genetics of this microorganism. Thus, our objective was to evaluate new markers for the study of the diversity and intra-taxa genetic structure of G. intestinalis in silico and in DNA obtained from stool samples. We analysed nine constitutive genes in 80 complete genome sequences and in a group of 24 stool samples from Colombia. Allelic diversity was evaluated by locus and for the concatenated sequence of nine loci that could discriminate up to 53 alleles. Phylogenetic reconstructions allowed us to identify AI, AII and B assemblages. We found evidence of intra- and inter-assemblage recombination events. Population structure analysis showed genetic differentiation among the assemblages analysed. View Full-Text
Keywords: G. intestinalis; genetic diversity; genetic structure; recombination G. intestinalis; genetic diversity; genetic structure; recombination
Show Figures

Figure 1

MDPI and ACS Style

Higuera, A.; Muñoz, M.; López, M.C.; Reyes, P.; Urbano, P.; Villalobos, O.; Ramírez, J.D. Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis. Genes 2020, 11, 764.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop