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Open AccessArticle

Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis

1
Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 111211, Colombia
2
Departamento de Salud Pública, Universidad Nacional de Colombia, Bogotá 111321, Colombia
3
Grupo de Investigaciones Biológicas de la Orinoquia, Unitrópico, Yopal 8500, Colombia
4
Hospital Local Santa María de Mompox, Programas Especiales (Lepra y TB), Mompox, Bolívar 132560, Colombia
*
Author to whom correspondence should be addressed.
Genes 2020, 11(7), 764; https://doi.org/10.3390/genes11070764
Received: 14 June 2020 / Revised: 27 June 2020 / Accepted: 28 June 2020 / Published: 8 July 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Giardia intestinalis is an intestinal protozoan most commonly found in humans. It has been grouped into 8 assemblages (A-H). Markers such as the glutamate dehydrogenase gene, triose phosphate isomerase and beta-giardin (β-giardin) have been widely used for genotyping. In addition, different genetic targets have been proposed as a valuable alternative to assess diversity and genetics of this microorganism. Thus, our objective was to evaluate new markers for the study of the diversity and intra-taxa genetic structure of G. intestinalis in silico and in DNA obtained from stool samples. We analysed nine constitutive genes in 80 complete genome sequences and in a group of 24 stool samples from Colombia. Allelic diversity was evaluated by locus and for the concatenated sequence of nine loci that could discriminate up to 53 alleles. Phylogenetic reconstructions allowed us to identify AI, AII and B assemblages. We found evidence of intra- and inter-assemblage recombination events. Population structure analysis showed genetic differentiation among the assemblages analysed. View Full-Text
Keywords: G. intestinalis; genetic diversity; genetic structure; recombination G. intestinalis; genetic diversity; genetic structure; recombination
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Higuera, A.; Muñoz, M.; López, M.C.; Reyes, P.; Urbano, P.; Villalobos, O.; Ramírez, J.D. Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis. Genes 2020, 11, 764.

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