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Analysis of Gene Expression of miRNA-106b-5p and TRAIL in the Apoptosis Pathway in Gastric Cancer

Marilia Medical School (FAMEMA), Marília, São Paulo 17519-030, Brazil
Department of Biosciences and Technology of Institute of Tropical Pathology and Public Health, Federal University of Goias (UFG), Goiânia, Goiás 74605-050, Brazil
Department of Morphology and Genetics, Escola Paulista de Medicina, Federal University of Sao Paulo (UNIFESP), São Paulo 04023-062, Brazil
Author to whom correspondence should be addressed.
Genes 2020, 11(4), 393;
Received: 28 January 2020 / Revised: 30 March 2020 / Accepted: 1 April 2020 / Published: 5 April 2020
(This article belongs to the Special Issue microRNAs in Tumor Microenvironments)
Helicobacter pylori (H. pylori) is one of the main causes of gastric gancer. TNF-related apoptosis-inducing ligand (TRAIL) is a protein able to promote apoptosis in cancer cells, however not in gastric cancer, which presents resistance to apoptosis via TRAIL. It is believed that MicroRNA-106b-5p might be involved in this resistance, although its role in Gastric Cancer is unclear. We aimed to determine the expression of microRNA-106b-5p and TRAIL in patients with gastric diseases, infected by H. pylori, and understand the relationship between these genes and their role in apoptosis and the gastric cancer pathways. H. pylori was detected by PCR, gene expression analysis was performed by real-time-qPCR, and bioinformatics analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cytoscape software. A total of 244 patients were divided into groups (Control, Gastritis, and Cancer); H. pylori was detected in 42.2% of the samples. The cancer group had a poor expression of TRAIL (p < 0.0001) and overexpression of microRNA-106b-5p (p = 0.0005), however, our results confirmed that these genes are not directly related to each other although both are apoptosis-related regulators. Our results also indicated that H. pylori decreases microRNA-106b-5p expression and that this is a carcinogenic bacterium responsible for gastric diseases. View Full-Text
Keywords: Helicobacter pylori; gastric cancer; apoptosis; TRAIL; microRNA-106b-5p Helicobacter pylori; gastric cancer; apoptosis; TRAIL; microRNA-106b-5p
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Pereira, J.; Santos, M.; Delabio, R.; Barbosa, M.; Smith, M.; Payão, S.; Rasmussen, L. Analysis of Gene Expression of miRNA-106b-5p and TRAIL in the Apoptosis Pathway in Gastric Cancer. Genes 2020, 11, 393.

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