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Open AccessArticle

Identification, Expression, and Functions of the Somatostatin Gene Family in Spotted Scat (Scatophagus argus)

by Peizhe Feng 1,2,†, Changxu Tian 1,2,3,†, Xinghua Lin 1,2, Dongneng Jiang 1,2,3, Hongjuan Shi 1,2,3, Huapu Chen 1,2,3, Siping Deng 1,2,3, Chunhua Zhu 1,2,3 and Guangli Li 1,2,3,*
1
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
2
Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Zhanjiang 524088, China
3
Marine Ecology and Aquaculture Environment of Zhanjiang, Zhanjiang 524088, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2020, 11(2), 194; https://doi.org/10.3390/genes11020194 (registering DOI)
Received: 28 October 2019 / Revised: 31 January 2020 / Accepted: 7 February 2020 / Published: 12 February 2020
(This article belongs to the Section Animal Genetics and Genomics)
Somatostatins (SSTs) are a family of proteins consisting of structurally diverse polypeptides that play important roles in the growth regulation in vertebrates. In the present study, four somatostatin genes (SST1, SST3, SST5, and SST6) were identified and characterized in the spotted scat (Scatophagus argus). The open reading frames (ORFs) of SST1, SST3, SST5, and SST6 cDNA consist of 372, 384, 321, and 333 bp, respectively, and encode proteins of 123, 127, 106, and 110 amino acids, respectively. Amino acid sequence alignments indicated that all SST genes contained conserved somatostatin signature motifs. Real-time PCR analysis showed that the SST genes were expressed in a tissue specific manner. When liver fragments were cultured in vitro with synthetic peptides (SST1, SST2, or SST6 at 1 μM or 10 μM) for 3 h or 6 h, the expression of insulin-like growth factor 1 and 2 (Igf-1 and Igf-2) in the liver decreased significantly. Treatment with SST5 had no significant effect on Igf-1 and Igf-2 gene expression. This study provides an enhanced understanding of the gene structure and expression patterns of the SST gene family in S. argus. Furthermore, this study provides a foundation for future exploration into the role of SST genes in growth and development.
Keywords: Scatophagus argus; somatostatin family; tissues expression; vitro incubation Scatophagus argus; somatostatin family; tissues expression; vitro incubation
MDPI and ACS Style

Feng, P.; Tian, C.; Lin, X.; Jiang, D.; Shi, H.; Chen, H.; Deng, S.; Zhu, C.; Li, G. Identification, Expression, and Functions of the Somatostatin Gene Family in Spotted Scat (Scatophagus argus). Genes 2020, 11, 194.

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