Next Article in Journal / Special Issue
Cohesin in Oocytes—Tough Enough for Mammalian Meiosis?
Previous Article in Journal / Special Issue
A Global Expression Switch Marks Pachytene Initiation during Mouse Male Meiosis
Open AccessArticle

The Mouse Cohesin-Associated Protein PDS5B Is Expressed in Testicular Cells and Is Associated with the Meiotic Chromosome Axes

Department of Cell and Molecular Biology, Karolinska Institute/Berzelius vag 35, 171-77 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Genes 2010, 1(3), 484-494; https://doi.org/10.3390/genes1030484
Received: 2 October 2010 / Revised: 18 November 2010 / Accepted: 1 December 2010 / Published: 13 December 2010
(This article belongs to the Special Issue Genetics of Mammalian Meiosis)
During the first meiotic prophase, the cohesin complex is localized to the chromosome axis and contributes to chromosome organization, pairing, synapsis, and recombination. The PDS5 protein, an accessory factor of the cohesin complex, is known to be a component of meiotic chromosome cores in fungi and to be implicated in meiotic chromosome structure and function. We found by immunoblotting experiments that a mammalian PDS5 protein, PDS5B, is abundantly expressed in mouse testis compared to other tissues. Immunofluorescence labeling experiments revealed that PDS5B is highly expressed in spermatogonia and that most PDS5B is depleted from chromatin as cells enter meiosis. During the first meiotic prophase, PDS5B associates with the axial cores of chromosomes. The axial association of PDS5B was observed also in the absence of synaptonemal complex proteins, such as SYCP1 and SYCP3, suggesting that PDS5B is an integral part of the chromosome axis as defined by the cohesin complex. These results suggest that PDS5B modulates cohesin functions in spermatocytes as well as in spermatogonia, contributing to meiotic chromosome structure and function. View Full-Text
Keywords: meiosis; chromosome; cohesin; PDS5; synaptonemal complex meiosis; chromosome; cohesin; PDS5; synaptonemal complex
Show Figures

Figure 1

MDPI and ACS Style

Fukuda, T.; Hoog, C. The Mouse Cohesin-Associated Protein PDS5B Is Expressed in Testicular Cells and Is Associated with the Meiotic Chromosome Axes. Genes 2010, 1, 484-494. https://doi.org/10.3390/genes1030484

AMA Style

Fukuda T, Hoog C. The Mouse Cohesin-Associated Protein PDS5B Is Expressed in Testicular Cells and Is Associated with the Meiotic Chromosome Axes. Genes. 2010; 1(3):484-494. https://doi.org/10.3390/genes1030484

Chicago/Turabian Style

Fukuda, Tomoyuki; Hoog, Christer. 2010. "The Mouse Cohesin-Associated Protein PDS5B Is Expressed in Testicular Cells and Is Associated with the Meiotic Chromosome Axes" Genes 1, no. 3: 484-494. https://doi.org/10.3390/genes1030484

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop