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Polymers
Volume 16
Issue 14
10.3390/polym16141968
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Article

The Effect of the Overexpression or Addition of IGF1 and FGF2 in Human Chondrocytes Included in a Fibrin Matrix and Cultivated in a Dynamic Environment

by
Jorge Lara-Arias
1,
Victor Manuel Peña-Martínez
1,
Luis Alejandro Rodriguez-Corpus
1,
Viktor J. Romero-Díaz
2,
Eduardo Álvarez-Lozano
1,* and
Herminia G. Martínez-Rodríguez
3,*
1
Orthopedics and Traumatology Service, Hospital Dr. José E. González, Universidad Autónoma de Nuevo León, Av. Dr. José Eleuterio González 235, Mitras Centro, Monterrey 64460, NL, Mexico
2
Histology Department, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Dr. José Eleuterio González 235, Mitras Centro, Monterrey 64460, NL, Mexico
3
Biochemistry and Molecular Medicine Department, Facultad de Medicina, Universidad Autónoma de Nuevo León, Av. Dr. José Eleuterio González 235, Mitras Centro, Monterrey 64460, NL, Mexico
*
Authors to whom correspondence should be addressed.
Polymers 2024, 16(14), 1968; https://doi.org/10.3390/polym16141968
Submission received: 22 May 2024 / Revised: 24 June 2024 / Accepted: 8 July 2024 / Published: 10 July 2024
(This article belongs to the Section Biobased and Biodegradable Polymers)
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Abstract

Hyaline cartilage is a highly specialized tissue. When injured, its repair capacity is low, which results in the massive destruction of the articular surface. Using tissue engineering and genetic engineering techniques, it is possible to provide a suitable microenvironment providing chondrocyte growth factors involved in the development of hyaline cartilage proteins, as well as cell proliferation and differentiation. Our aim was to stimulate the synthesis of an extracellular matrix via the chondrocytes included in a fibrin matrix through the addition or overexpression of IGF1 and/or FGF2, while maintaining a constant agitation of the culture medium. Collagen type II and glycosaminoglycans increased during the entire incubation time. In contrast, collagen type I decreased its expression under the same culture conditions, transfecting or supplementing growth factors to chondrocytes. However, chondrocytes that were not transfected or supplemented showed a general increase in the proteins analyzed in this study. The presence of IGF1 and FGF2 increased the protein synthesis of the hyaline cartilage, regardless of which one was the source of growth factors. Continuous agitation using the spinner flask allows for the adequate nutrition of chondrocytes included in the fibrin matrix. However, they require growth factors to up-regulate or down-regulate collagenous proteins.
Keywords: tissue engineering; dynamic culture; transfected chondrocytes; growth factors tissue engineering; dynamic culture; transfected chondrocytes; growth factors
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MDPI and ACS Style

Lara-Arias, J.; Peña-Martínez, V.M.; Rodriguez-Corpus, L.A.; Romero-Díaz, V.J.; Álvarez-Lozano, E.; Martínez-Rodríguez, H.G. The Effect of the Overexpression or Addition of IGF1 and FGF2 in Human Chondrocytes Included in a Fibrin Matrix and Cultivated in a Dynamic Environment. Polymers 2024, 16, 1968. https://doi.org/10.3390/polym16141968

AMA Style

Lara-Arias J, Peña-Martínez VM, Rodriguez-Corpus LA, Romero-Díaz VJ, Álvarez-Lozano E, Martínez-Rodríguez HG. The Effect of the Overexpression or Addition of IGF1 and FGF2 in Human Chondrocytes Included in a Fibrin Matrix and Cultivated in a Dynamic Environment. Polymers. 2024; 16(14):1968. https://doi.org/10.3390/polym16141968

Chicago/Turabian Style

Lara-Arias, Jorge, Victor Manuel Peña-Martínez, Luis Alejandro Rodriguez-Corpus, Viktor J. Romero-Díaz, Eduardo Álvarez-Lozano, and Herminia G. Martínez-Rodríguez. 2024. "The Effect of the Overexpression or Addition of IGF1 and FGF2 in Human Chondrocytes Included in a Fibrin Matrix and Cultivated in a Dynamic Environment" Polymers 16, no. 14: 1968. https://doi.org/10.3390/polym16141968

APA Style

Lara-Arias, J., Peña-Martínez, V. M., Rodriguez-Corpus, L. A., Romero-Díaz, V. J., Álvarez-Lozano, E., & Martínez-Rodríguez, H. G. (2024). The Effect of the Overexpression or Addition of IGF1 and FGF2 in Human Chondrocytes Included in a Fibrin Matrix and Cultivated in a Dynamic Environment. Polymers, 16(14), 1968. https://doi.org/10.3390/polym16141968

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