Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration
Abstract
:1. Introduction
2. Materials and Methods
Assessment of the Degree of Nerve Conduction Recovery
3. Results
3.1. Physical and Mechanical Characteristics of Chitosan Fibers
3.2. Biocompatibility of Conduits
3.3. Nerve Conduction Recovery Assessment
3.4. Motor Recovery Assessment
3.5. Investigation of Motor-Coordination Disorders
3.6. Neuromuscular Function Recovery Assessment
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Fibers | Tensile Strength, σ, MPa | Young Modulus, GPa | Elongation at Break, ε, % |
---|---|---|---|
MChs | 240.96 ± 7.98 | 13.83 ± 0.54 | 6.3 ± 0.72 |
PChs | 186.58 ± 19.91 | 12.09 ± 1.9 | 3.36 ± 0.32 |
PLA/Chs-ChNF 0.5% | 259.31 ± 10.45 | 14.41 ± 0.73 | 5.35 ± 0.34 |
PLA/Chs-ChNF 30% | 174.37 ± 7.31 | 14.01 ± 0.81 | 3.87 ± 0.43 |
PLA/Chs-ChNF 50% | 163.86 ± 9.26 | 13.87 ± 0.19 | 2.57 ± 0.55 |
Fibers | Diameter, µm | ||||||
---|---|---|---|---|---|---|---|
Dry | 3 Days | 7 Days | 14 Days | 30 Days | 60 Days | 90 Days | |
PChs | 24.17 ± 4.19 | 35.52 ± 3.91 | 37.55 ± 2.90 | 42.16 ± 3.99 | 65.37 ± 8.15 | 68.92 ± 5.21 | 70.34 ± 8.13 |
MChs | 43.67 ± 3.79 | 52.67 ± 5.59 | 60.38 ± 5.21 | 78.23 ± 7.89 | 101.37 ± 7.73 | 102.72 ± 7.01 | 103.44 ± 6.93 |
Chs-ChNF 0.5% | 42.75 ± 2.24 | 47.16 ± 3.67 | 59.74 ± 4.02 | 69.10 ± 3.72 | 95.48 ± 2.31 | 96.63 ± 6.29 | 98.04 ± 5.90 |
Chs-ChNF 30% | 40.88 ± 1.98 | 60.01 ± 4.97 | 70.16 ± 3.18 | 83.87 ± 4.71 | 91.92 ± 5.01 | 93.51 ± 4.61 | 93.34 ± 4.01 |
Chs-ChNF 50% | 43.10 ± 2.03 | 55.82 ± 3.72 | 75.20 ± 2.94 | 80.42 ± 4.71 | 90.15 ± 2.59 | 89.89 ± 4.75 | 91.56 ± 3.09 |
Conduit | M-Response Amplitude (mV) | ||
---|---|---|---|
Intact Nerve | 4 Weeks | 16 Weeks | |
AG | 28.9 ± 3.3 | 6.8 ± 2.4 | 15.1 ± 5.2 |
PLA | 27.3 ± 3.8 | 5.1 ± 1.8 | 8.8 ± 3.9 |
PLA/MChs | 27.2 ± 3.6 | 5.5 ± 2.2 | 13.8 ± 3.2 |
PLA/PChs | 27.8 ± 2.1 | 3.9 ± 1.5 | 6.9 ± 4.7 |
PLA/Chs-ChNF 0.5% | 30.1 ± 3.1 | 4.3 ± 1.7 | 12.5 ± 5.8 |
PLA/Chs-ChNF 30% | 28.8 ± 3.7 | 4.9 ± 1.4 | 12.7 ± 3.7 |
PLA/Chs-ChNF 50% | 28.2 ± 2.5 | 5.3 ± 0.9 | 14.1 ± 4.6 |
Type if Conduit | Grip Force, N | ||
---|---|---|---|
Intact Nerve | Conduit | Recovery Degree, % | |
AG | 2.77 ± 0.41 (14%) | 1.54 ± 0.21 (13%) | 55 |
PLA | 1.80 ± 0.17 (10%) | 0.59 ± 0.1 (17%) | 33 |
MChs | 2.54 ± 0.31 (12%) | 1.14 ± 0.41 (36%) | 45 |
PChs | - | - | - |
Chs-ChNF 0.5% | 1.95 ± 0.49 (25%) | 0.82 ± 0.17 (21%) | 42 |
Chs-ChNF 30% | 2.06 ± 0.31 (15%) | 0.96 ± 0.25 (26%) | 46 |
Chs-ChNF 50% | 2.73 ± 0.51 (19%) | 1.20 ± 0.48 (40%) | 44 |
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Tagandurdyyeva, N.A.; Trube, M.A.; Shemyakin, I.O.; Solomitskiy, D.N.; Medvedev, G.V.; Dresvyanina, E.N.; Nashchekina, Y.A.; Ivan’kova, E.M.; Dobrovol’skaya, I.P.; Kamalov, A.M.; et al. Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration. Polymers 2023, 15, 3323. https://doi.org/10.3390/polym15153323
Tagandurdyyeva NA, Trube MA, Shemyakin IO, Solomitskiy DN, Medvedev GV, Dresvyanina EN, Nashchekina YA, Ivan’kova EM, Dobrovol’skaya IP, Kamalov AM, et al. Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration. Polymers. 2023; 15(15):3323. https://doi.org/10.3390/polym15153323
Chicago/Turabian StyleTagandurdyyeva, Nurjemal A., Maxim A. Trube, Igor’ O. Shemyakin, Denis N. Solomitskiy, German V. Medvedev, Elena N. Dresvyanina, Yulia A. Nashchekina, Elena M. Ivan’kova, Irina P. Dobrovol’skaya, Almaz M. Kamalov, and et al. 2023. "Properties of Resorbable Conduits Based on Poly(L-Lactide) Nanofibers and Chitosan Fibers for Peripheral Nerve Regeneration" Polymers 15, no. 15: 3323. https://doi.org/10.3390/polym15153323