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Article

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

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Centre for Rapid and Sustainable Product Development (CDRSP), Polytechnic of Leiria, Marinha Grande, 2430-028 Leiria, Portugal
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Department of Bioengineering and IBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
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Associate Laboratory I4HB—Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Roman A. Surmenev
Polymers 2022, 14(9), 1669; https://doi.org/10.3390/polym14091669
Received: 19 February 2022 / Revised: 21 March 2022 / Accepted: 14 April 2022 / Published: 20 April 2022
(This article belongs to the Special Issue Polymeric Materials for Biomedical Applications)
Understanding the mechano–biological coupling mechanisms of biomaterials for tissue engineering is of major importance to assure proper scaffold performance in situ. Therefore, it is of paramount importance to establish correlations between biomaterials, their processing conditions, and their mechanical behaviour, as well as their biological performance. With this work, it was possible to infer a correlation between the addition of graphene nanoparticles (GPN) in a concentration of 0.25, 0.5, and 0.75% (w/w) (GPN0.25, GPN0.5, and GPN0.75, respectively) in three-dimensional poly(ε-caprolactone) (PCL)-based scaffolds, the extrusion-based processing parameters, and the lamellar crystal orientation through small-angle X-ray scattering experiments of extruded samples of PCL and PCL/GPN. Results revealed a significant impact on the scaffold’s mechanical properties to a maximum of 0.5% of GPN content, with a significant improvement in the compressive modulus of 59 MPa to 93 MPa. In vitro cell culture experiments showed the scaffold’s ability to support the adhesion and proliferation of L929 fibroblasts (fold increase of 28, 22, 23, and 13 at day 13 (in relation to day 1) for PCL, GPN0.25, GPN0.5, and GPN0.75, respectively) and bone marrow mesenchymal stem/stromal cells (seven-fold increase for all sample groups at day 21 in relation to day 1). Moreover, the cells maintained high viability, regular morphology, and migration capacity in all the different experimental groups, assuring the potential of PCL/GPN scaffolds for tissue engineering (TE) applications. View Full-Text
Keywords: additive manufacturing; graphene; lamellar crystal orientation; mechanical properties; poly(ε-caprolactone); tissue engineering additive manufacturing; graphene; lamellar crystal orientation; mechanical properties; poly(ε-caprolactone); tissue engineering
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MDPI and ACS Style

Biscaia, S.; Silva, J.C.; Moura, C.; Viana, T.; Tojeira, A.; Mitchell, G.R.; Pascoal-Faria, P.; Ferreira, F.C.; Alves, N. Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance. Polymers 2022, 14, 1669. https://doi.org/10.3390/polym14091669

AMA Style

Biscaia S, Silva JC, Moura C, Viana T, Tojeira A, Mitchell GR, Pascoal-Faria P, Ferreira FC, Alves N. Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance. Polymers. 2022; 14(9):1669. https://doi.org/10.3390/polym14091669

Chicago/Turabian Style

Biscaia, Sara, João C. Silva, Carla Moura, Tânia Viana, Ana Tojeira, Geoffrey R. Mitchell, Paula Pascoal-Faria, Frederico C. Ferreira, and Nuno Alves. 2022. "Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance" Polymers 14, no. 9: 1669. https://doi.org/10.3390/polym14091669

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