Constraint Release for Reptating Filaments in Semiflexible Networks Depends on Background Fluctuations
Abstract
:1. Introduction
2. Materials and Methods
2.1. F-Actin and Actin Crosslinker wLX
2.2. DNA Nanotubes as Tracers
2.3. Sample Preparation and Measurement
2.4. Data Analysis
2.5. Significance Test/Wilcoxon Rank-Sum Test
3. Results
3.1. MSD of Transverse Fluctuations
3.2. Entanglement Times
4. Discussion
4.1. Constraint Release in Entangled F-Actin Networks
4.2. Entanglement Time Dependency on Tracer Persistence Length
4.3. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
nHT | n-helix tube |
wLX | weak LifeAct®-based crosslinker |
MSD | mean squared displacement |
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Händler, T.; Tutmarc, C.; Freitag, J.S.; Smith, D.M.; Schnauß, J. Constraint Release for Reptating Filaments in Semiflexible Networks Depends on Background Fluctuations. Polymers 2022, 14, 707. https://doi.org/10.3390/polym14040707
Händler T, Tutmarc C, Freitag JS, Smith DM, Schnauß J. Constraint Release for Reptating Filaments in Semiflexible Networks Depends on Background Fluctuations. Polymers. 2022; 14(4):707. https://doi.org/10.3390/polym14040707
Chicago/Turabian StyleHändler, Tina, Cary Tutmarc, Jessica S. Freitag, David M. Smith, and Jörg Schnauß. 2022. "Constraint Release for Reptating Filaments in Semiflexible Networks Depends on Background Fluctuations" Polymers 14, no. 4: 707. https://doi.org/10.3390/polym14040707