Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification
Abstract
:1. Introduction
2. Materials and Methods
2.1. Raw Material
2.2. Aspen Wood Delignification and Extraction of the Hemicelluloses
2.3. Gel Permeation Chromatography
2.4. Analysis of the Monosaccharide Composition
2.5. Fourier-Transform Infra-Red Spectroscopy
2.6. Nuclear Magnetic Resonance
2.7. Thermogravimetric Analysis
2.8. Optimization
2.9. Antioxidant Activity
2.9.1. DPPH Radical Scavenging Assay
2.9.2. Hydroxyl Radical Scavenging Assay
3. Results
3.1. Hemicellulose Delignification and Yield
3.2. Optimization of the Oxidative Delignification
3.3. Gel Permeation Chromatography Study of the Hemicelluloses
3.4. Analysis of the Monosaccharide Composition of the Hemicelluloses
3.5. Fourier-Transform Infra-Red Spectroscopy
3.6. Nuclear Magnetic Resonance
3.7. Thermogravimetric Analysis
3.8. Analysis of the Antioxidant Activity of the Hemicelluloses
3.8.1. Scavenging Activity of the DPPH Radicals
3.8.2. Scavenging Activity of the Hydroxyl Radicals
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Process Temperatures (°C) | 70 | 80 | 90 | 100 |
---|---|---|---|---|
Process Times (h) | ||||
4 | + | + | + | + |
3 | - | + | + | + |
2 | - | - | - | + |
1 | - | - | - | + |
Process Temperatures (°C) | 70 | 80 | 90 | 100 |
---|---|---|---|---|
Process Times (h) | Hemicellulose Yield (wt%) 1 | |||
4 | 0.67 2 | 1.81 3 | 4.79 5 | 9.68 8 |
3 | - | 0.92 4 | 2.83 6 | 7.08 9 |
2 | - | - | 1.09 7 | 6.30 10 |
1 | - | - | - | 4.92 11 |
Factors and Parameters | Designations | |
---|---|---|
In the Text and Figures | In the Equations | |
Temperature (°C) | T | X1 |
Time (h) | t | X2 |
Hemicellulose yield (%) | Yield | Y1 |
Polydispersity | PD | Y2 |
Sample | Reaction Temperature (°C) | Reaction Time (h) | Characteristics | |
---|---|---|---|---|
Yield, Mass % | PDI | |||
- | X1 (T) | X2 (t) | Y1 | Y2 |
80-3 | 80 | 3 | 0.973 | 5.22 |
90-3 | 90 | 3 | 2.833 | 2.94 |
100-3 | 100 | 3 | 7.075 | 1.92 |
80-4 | 80 | 4 | 1.812 | 3.53 |
90-4 | 90 | 4 | 4.785 | 2.10 |
100-4 | 100 | 4 | 9.678 | 2.05 |
Variance Sources | Output Parameters | |||
---|---|---|---|---|
Yield Y1, Mass % | Polydispersity, Y2 | |||
Statistical Characteristics | ||||
Variance Relations F | Significance Levels p | Variance Relations F | Significance Levels p | |
X1: T | 1096.8 | 0.0113 | 4760.1 | 0.0092 |
X2: Prod | 160.6 | 0.0355 | 800.0 | 0.0225 |
X12 | 48.8 | 0.0642 | 484.0 | 0.0289 |
X1X2 | 23.7 | 0.0918 | 690.1 | 0.0242 |
Number of degrees of freedom | 2 | 2 | ||
R2adj | 99.6 | 99.9 |
Sample | Mw (g/mol) | PDI |
---|---|---|
70-4 | 33,142 | 6.175 |
80-4 | 20,266 | 3.528 |
90-4 | 14,149 | 2.102 |
100-4 | 8932 | 2.050 |
Sample | Mw (g/mol) | PDI |
---|---|---|
100-4 | 8932 | 2.050 |
100-3 | 11,228 | 1.918 |
100-2 | 10,290 | 1.926 |
100-1 | 10,484 | 1.988 |
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Borovkova, V.S.; Malyar, Y.N.; Sudakova, I.G.; Chudina, A.I.; Zimonin, D.V.; Skripnikov, A.M.; Miroshnikova, A.V.; Ionin, V.A.; Kazachenko, A.S.; Sychev, V.V.; et al. Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification. Polymers 2022, 14, 4521. https://doi.org/10.3390/polym14214521
Borovkova VS, Malyar YN, Sudakova IG, Chudina AI, Zimonin DV, Skripnikov AM, Miroshnikova AV, Ionin VA, Kazachenko AS, Sychev VV, et al. Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification. Polymers. 2022; 14(21):4521. https://doi.org/10.3390/polym14214521
Chicago/Turabian StyleBorovkova, Valentina S., Yuriy N. Malyar, Irina G. Sudakova, Anna I. Chudina, Dmitriy V. Zimonin, Andrey M. Skripnikov, Angelina V. Miroshnikova, Vladislav A. Ionin, Alexander S. Kazachenko, Valentin V. Sychev, and et al. 2022. "Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification" Polymers 14, no. 21: 4521. https://doi.org/10.3390/polym14214521