Attenuation of Pseudomonas aeruginosa Quorum Sensing Virulence of Biofilm and Pyocyanin by mBTL-Loaded Calcium Alginate Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Growth Conditions
2.2. Growth Curve of Bacterial Isolates
2.3. Biofilm Formation Assay
2.4. Pyocyanin Production Assay
2.5. Cytotoxicity Studies
2.6. Bacterial Adhesion Assay
2.7. Statistical Analysis
3. Results
3.1. Growth Pattern of P. aeruginosa QS Mutants Challenged with Free mBTL and mBTL-Loaded CANPs
3.2. Effect of mBTL and mBTL-Loaded CANPs on Bacterial Biofilm Formation
3.3. Effect of mBTL and mBTL-Loaded CANPs on Bacterial Pyocyanin Production
3.4. Cytotoxicity of Formulated CANPs
3.5. Effect of mBTL-Loaded CANPs on the Adhesion of P. aeruginosa to Human Lung A549 Cell Line
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bacterial Strain | Abbreviation | Description | Reference |
---|---|---|---|
PA01 | PA01 | PA01 wild-type | [21] |
PA1430 | ΔlasR | Transcription regulator LasR | [21] |
PA1432 | ΔlasI | Autoinducer synthesis protein LasI | [21] |
PA1871 | ΔlasA | LasA protease precursor | [21] |
PA3477 | ΔrhIR | Transcriptional regulator RhIR | [21] |
D6A | ΔlasR ΔrhlR double mutant | ΔlasR ΔrhlR knockouts double mutant | [21] |
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Eltayb, E.K.; Alqahtani, F.Y.; Alkahtani, H.M.; Alsarra, I.A.; Alfaraj, R.; Aleanizy, F.S. Attenuation of Pseudomonas aeruginosa Quorum Sensing Virulence of Biofilm and Pyocyanin by mBTL-Loaded Calcium Alginate Nanoparticles. Polymers 2022, 14, 3655. https://doi.org/10.3390/polym14173655
Eltayb EK, Alqahtani FY, Alkahtani HM, Alsarra IA, Alfaraj R, Aleanizy FS. Attenuation of Pseudomonas aeruginosa Quorum Sensing Virulence of Biofilm and Pyocyanin by mBTL-Loaded Calcium Alginate Nanoparticles. Polymers. 2022; 14(17):3655. https://doi.org/10.3390/polym14173655
Chicago/Turabian StyleEltayb, Esra Kamal, Fulwah Yahya Alqahtani, Hamad M. Alkahtani, Ibrahim A. Alsarra, Rihaf Alfaraj, and Fadilah Sfouq Aleanizy. 2022. "Attenuation of Pseudomonas aeruginosa Quorum Sensing Virulence of Biofilm and Pyocyanin by mBTL-Loaded Calcium Alginate Nanoparticles" Polymers 14, no. 17: 3655. https://doi.org/10.3390/polym14173655