Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparing of AgNPs and PMCs
- 1.
- Ex Situ prepared PVA-5AgNPs composite—to prepare the PVA-5AgNPs composite 8 wt.% solution of PVA and AgNPs were mixed together:
- (a)
- Preparing of 8 wt.% PVA solution—PVA powder and deionized water were mixed in a beaker, in a water bath at 70–80 °C for 2 h,
- (b)
- Synthesis of AgNPs—chemicals for experiment included the stock silver solution and leaves extract of R. officinalis:
- (i)
- the stock silver solution was prepared by dissolving of AgNO3 in deionized water (concentration of solution 50 mg/L Ag),
- (ii)
- the leaf extract was prepared using 10 g of fresh leaves of R. officinalis. Cleaned leaves were ground and mixed with 125 mL of deionized water, heated at 70 °C for 10 min, filtered, and centrifuged to remove solid residue; 100 mL of pure leaf extract was obtained,
- (iii)
- for the synthesis of AgNPs 20 mL of extract was added dropwise to 80 mL of stock silver solution heated to 70–80 °C. The pre-prepared 100 mL of AgNPs colloidal solution was thickened by centrifuge (3 mL of colloidal AgNPs was received).
- 2.
- In Situ prepared PVA-5AgNPs composite-PVA powder, AgNO3 water solution, and R. officinalis leaf extract were mixed simultaneously in a beaker by magnetic stirrer at 80 °C in a water bath for 3 h. The amounts of the individual components were adjusted so that the concentration of PVA and Ag was the same as in the ex situ preparation process.
2.3. Methods of Measurements
2.4. Antibiofilm Activity
3. Results and Discussion
3.1. Synthesis and Characterization of Pre-Prepared Silver Nanoparticles
3.2. Characterization of Polymer Matrix Composite
3.3. Antibiofilm Effect
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Velgosova, O.; Mudra, E.; Vojtko, M. Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs. Polymers 2021, 13, 605. https://doi.org/10.3390/polym13040605
Velgosova O, Mudra E, Vojtko M. Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs. Polymers. 2021; 13(4):605. https://doi.org/10.3390/polym13040605
Chicago/Turabian StyleVelgosova, Oksana, Erika Mudra, and Marek Vojtko. 2021. "Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs" Polymers 13, no. 4: 605. https://doi.org/10.3390/polym13040605