Next Article in Journal
How Microgels Can Improve the Impact of Organ-on-Chip and Microfluidic Devices for 3D Culture: Compartmentalization, Single Cell Encapsulation and Control on Cell Fate
Next Article in Special Issue
Nature-Based Biomaterials and Their Application in Biomedicine
Previous Article in Journal
Relaxation Phenomena in Chitosan-Au Nanoparticle Thin Films
Previous Article in Special Issue
Current Insights into Collagen Type I
Communication

Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications

1
School of Engineering and Digital Sciences, Nazarbayev University, Nur-Sultan 010000, Kazakhstan
2
School of Sciences and Humanities, Nazarbayev University, Nur-Sultan 010000, Kazakhstan
*
Author to whom correspondence should be addressed.
Academic Editors: Dimitrios Bikiaris and Andrea Ehrmann
Polymers 2021, 13(19), 3215; https://doi.org/10.3390/polym13193215
Received: 1 August 2021 / Revised: 12 September 2021 / Accepted: 15 September 2021 / Published: 23 September 2021
(This article belongs to the Special Issue Polymeric Biomaterials for Biomedical Applications)
The drug development process can greatly benefit from liver-on-a-chip platforms aiming to recapitulate the physiology, mechanisms, and functionalities of liver cells in an in vitro environment. The liver is the most important organ in drug metabolism investigation. Here, we report the development of a hybrid cyclic olefin copolymer (COC) and polydimethylsiloxane (PDMS) microfluidic (HCP) platform to culture a Huh7 hepatoma cell line in dynamic conditions towards the development of a liver-on-a-chip system. The microfluidic platform is comprised of a COC bottom layer with a microchannel and PDMS-based flat top layer sandwiched together. The HCP device was applied for culturing Huh7 cells grown on a collagen-coated microchannel. A computational fluid dynamics modeling study was conducted for the HCP device design revealing the presence of air volume fraction in the chamber and methods for optimizing experimental handling of the device. The functionality and metabolic activity of perfusion culture were assessed by the secretion rates of albumin, urea, and cell viability visualization. The HCP device hepatic culture remained functional and intact for 24 h, as assessed by resulting levels of biomarkers similar to published studies on other in vitro and 2D cell models. The present results provide a proof-of-concept demonstration of the hybrid COC–PDMS microfluidic chip for successfully culturing a Huh7 hepatoma cell line, thus paving the path towards developing a liver-on-a-chip platform. View Full-Text
Keywords: microfluidics; microfluidic chip; liver-on-a-chip; microphysiological platform; hepatocytes; huh7; cyclic olefin copolymer; polydimethylsiloxane; hybrid polymer-based liver-on-a-chip microfluidics; microfluidic chip; liver-on-a-chip; microphysiological platform; hepatocytes; huh7; cyclic olefin copolymer; polydimethylsiloxane; hybrid polymer-based liver-on-a-chip
Show Figures

Graphical abstract

MDPI and ACS Style

Kulsharova, G.; Kurmangaliyeva, A.; Darbayeva, E.; Rojas-Solórzano, L.; Toxeitova, G. Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications. Polymers 2021, 13, 3215. https://doi.org/10.3390/polym13193215

AMA Style

Kulsharova G, Kurmangaliyeva A, Darbayeva E, Rojas-Solórzano L, Toxeitova G. Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications. Polymers. 2021; 13(19):3215. https://doi.org/10.3390/polym13193215

Chicago/Turabian Style

Kulsharova, Gulsim, Akbota Kurmangaliyeva, Elvira Darbayeva, Luis Rojas-Solórzano, and Galiya Toxeitova. 2021. "Development of a Hybrid Polymer-Based Microfluidic Platform for Culturing Hepatocytes towards Liver-on-a-Chip Applications" Polymers 13, no. 19: 3215. https://doi.org/10.3390/polym13193215

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop