Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties
AbstractA straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides EPS B3-15 and EPS T14 and the poly-γ-glutamic acid γ-PGA-APA were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV−Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa), as well as fungi (Candida albicans) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against E. coli, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV–Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for EPS B3-15-Ag NPs and EPS T14-Ag NPs (Minimum Inhibitory Concentration, MIC: 9.37–45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25–75 µg/mL). View Full-Text
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Scala, A.; Piperno, A.; Hada, A.; Astilean, S.; Vulpoi, A.; Ginestra, G.; Marino, A.; Nostro, A.; Zammuto, V.; Gugliandolo, C. Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties. Polymers 2019, 11, 1157.
Scala A, Piperno A, Hada A, Astilean S, Vulpoi A, Ginestra G, Marino A, Nostro A, Zammuto V, Gugliandolo C. Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties. Polymers. 2019; 11(7):1157.Chicago/Turabian Style
Scala, Angela; Piperno, Anna; Hada, Alexandru; Astilean, Simion; Vulpoi, Adriana; Ginestra, Giovanna; Marino, Andreana; Nostro, Antonia; Zammuto, Vincenzo; Gugliandolo, Concetta. 2019. "Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties." Polymers 11, no. 7: 1157.
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