Next Article in Journal
Dissolved Gases Forecasting Based on Wavelet Least Squares Support Vector Regression and Imperialist Competition Algorithm for Assessing Incipient Faults of Transformer Polymer Insulation
Next Article in Special Issue
Polymer Translocation Across a Corrugated Channel: Fick–Jacobs Approximation Extended Beyond the Mean First-Passage Time
Previous Article in Journal
Polyelectrolyte Complexation of Oligonucleotides by Charged Hydrophobic—Neutral Hydrophilic Block Copolymers
Previous Article in Special Issue
Inferring Active Noise Characteristics from the Paired Observations of Anomalous Diffusion

Clog and Release, and Reverse Motions of DNA in a Nanopore

Department of Mathematics and Physics, Aoyama-Gakuin University, Sagamihara Campus L617, 5-10-1 Fuchinobe, Chuo, Sagamihara, Kanagawa 252-5258, Japan
Author to whom correspondence should be addressed.
Polymers 2019, 11(1), 84;
Received: 24 November 2018 / Revised: 28 December 2018 / Accepted: 3 January 2019 / Published: 7 January 2019
(This article belongs to the Special Issue Polymer Translocation)
Motions of circular and linear DNA molecules of various lengths near a nanopore of 100 or 200 nm diameter were experimentally observed and investigated by fluorescence microscopy. The movement of DNA molecules through nanopores, known as translocation, is mainly driven by electric fields near and inside the pores. We found significant clogging of nanopores by DNA molecules, particularly by circular DNA and linear T4 DNA (165.65 kbp). Here, the probabilities of DNA clogging events, depending on the DNA length and shape—linear or circular—were determined. Furthermore, two distinct DNA motions were observed: clog and release by linear T4 DNA, and a reverse direction motion at the pore entrance by circular DNA, after which both molecules moved away from the pore. Finite element method-based numerical simulations were performed. The results indicated that DNA molecules with pores 100–200 nm in diameter were strongly influenced by opposing hydrodynamic streaming flow, which was further enhanced by bulky DNA configurations. View Full-Text
Keywords: translocation; nanopore; DNA; electro-osmosis translocation; nanopore; DNA; electro-osmosis
Show Figures

Figure 1

MDPI and ACS Style

Kubota, T.; Lloyd, K.; Sakashita, N.; Minato, S.; Ishida, K.; Mitsui, T. Clog and Release, and Reverse Motions of DNA in a Nanopore. Polymers 2019, 11, 84.

AMA Style

Kubota T, Lloyd K, Sakashita N, Minato S, Ishida K, Mitsui T. Clog and Release, and Reverse Motions of DNA in a Nanopore. Polymers. 2019; 11(1):84.

Chicago/Turabian Style

Kubota, Tomoya, Kento Lloyd, Naoto Sakashita, Seiya Minato, Kentaro Ishida, and Toshiyuki Mitsui. 2019. "Clog and Release, and Reverse Motions of DNA in a Nanopore" Polymers 11, no. 1: 84.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop