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Open AccessArticle

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

1
Center for Natural and Human Sciences, Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP, CEP 09210-580, Brazil
2
Nanomedicine Research Unit (NANOMED), Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP 09210-580, Brazil
3
Tropical Disease Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP 13083-862, Brazil
4
Center for Engineering, Modeling and Applied Social Science, Universidade Federal do ABC, Alameda da Universidade sem numero, São Bernardo do Campo, SP, CEP 09606-045, Brazil
*
Author to whom correspondence should be addressed.
Polymers 2018, 10(4), 452; https://doi.org/10.3390/polym10040452
Received: 16 March 2018 / Revised: 8 April 2018 / Accepted: 16 April 2018 / Published: 18 April 2018
(This article belongs to the Collection Polysaccharides)
Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S-nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL)-chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent toxicity to Vero cells, and antimicrobial activity to Pseudomonas aeruginosa (minimum inhibitory concentration and minimum bactericidal concentration values of 0.5 µg·mL−1 of hydrogel, which corresponds to 1 mmol·L−1 of GSNO). Interestingly, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Thus, the GSNO-PL/CS hydrogel is a suitable biomaterial for topical NO delivery applications. View Full-Text
Keywords: chitosan; thermoresponsive hydrogel; nitric oxide; S-nitrosothiols; biocompatibility; antimicrobial; Pseudomonas aeruginosa; Pluronic F127 chitosan; thermoresponsive hydrogel; nitric oxide; S-nitrosothiols; biocompatibility; antimicrobial; Pseudomonas aeruginosa; Pluronic F127
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MDPI and ACS Style

Pelegrino, M.T.; De Araujo Lima, B.; Do Nascimento, M.H.M.; Lombello, C.B.; Brocchi, M.; Seabra, A.B. Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications. Polymers 2018, 10, 452.

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