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Toxins 2017, 9(8), 233;

Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses

Laboratory of Genetics, Institute of Botany, Nature Research Centre, Akademijos str. 2, Vilnius LT-08412, Lithuania
Department of Biochemistry and Molecular Biology, Institute of Biosciences, Vilnius University, Saulėtekio al. 7, Vilnius LT-10257, Lithuania
Life Science Research Centre and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic
Author to whom correspondence should be addressed.
Academic Editor: Manfred J. Schmitt
Received: 30 June 2017 / Revised: 17 July 2017 / Accepted: 21 July 2017 / Published: 25 July 2017
(This article belongs to the Special Issue Yeast Killer Toxins)
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Competitive and naturally occurring yeast killer phenotype is governed by coinfection with dsRNA viruses. Long-term relationship between the host cell and viruses appear to be beneficial and co-adaptive; however, the impact of viral dsRNA on the host gene expression has barely been investigated. Here, we determined the transcriptomic profiles of the host Saccharomyces cerevisiae upon the loss of the M-2 dsRNA alone and the M-2 along with the L-A-lus dsRNAs. We provide a comprehensive study based on the high-throughput RNA-Seq data, Gene Ontology and the analysis of the interaction networks. We identified 486 genes differentially expressed after curing yeast cells of the M-2 dsRNA and 715 genes affected by the elimination of both M-2 and L-A-lus dsRNAs. We report that most of the transcriptional responses induced by viral dsRNAs are moderate. Differently expressed genes are related to ribosome biogenesis, mitochondrial functions, stress response, biosynthesis of lipids and amino acids. Our study also provided insight into the virus–host and virus–virus interplays. View Full-Text
Keywords: Saccharomyces cerevisiae; dsRNA viruses; host gene expression; RNA-Seq Saccharomyces cerevisiae; dsRNA viruses; host gene expression; RNA-Seq

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Lukša, J.; Ravoitytė, B.; Konovalovas, A.; Aitmanaitė, L.; Butenko, A.; Yurchenko, V.; Serva, S.; Servienė, E. Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses. Toxins 2017, 9, 233.

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