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Toxins 2017, 9(10), 301; https://doi.org/10.3390/toxins9100301

Safety Assessment of Lactobacillus helveticus KLDS1.8701 Based on Whole Genome Sequencing and Oral Toxicity Studies

1
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China
2
Food College, Northeast Agricultural University, Harbin 150030, China
3
Food Science and Nutrition Unit, Department of Animal Science, Faculty of Agriculture, University of Benin, PMB 1154, Benin City 300001, Nigeria
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Sang Ho Choi
Received: 2 August 2017 / Revised: 4 September 2017 / Accepted: 20 September 2017 / Published: 24 September 2017
(This article belongs to the Section Bacterial Toxins)
Full-Text   |   PDF [877 KB, uploaded 24 September 2017]   |  

Abstract

Lactobacillus helveticus KLDS1.8701 isolated from Chinese traditional fermented dairy product has been shown earlier to possess probiotic potentials but it is important to evaluate its safety in view of its possible use as a probiotic. The aim of the present study is to critically assess the safety of L. helveticus KLDS1.8701 through multiple perspectives. The complete genome of L. helveticus KLDS1.8701 was sequenced to mine for safety-associated genes. The minimum inhibitory concentrations of 15 antimicrobials and the adverse metabolites were determined. Standard acute oral and subacute toxicity studies were conducted in rats. The results in silico disclosed that the genome of L. helveticus KLDS1.8701 carries no transferable antibiotic resistance genes, no virulence factors and only 3 genes related to adverse metabolites. In vitro results showed that L. helveticus KLDS1.8701 was resistant against 6 antimicrobials and did not raise safety concerns about biogenic amine, D-lactic acid and nitroreductase. The results in vivo revealed that no adverse effects on experimental rats were observed in the oral toxicity tests. Overall, findings from this study suggest that L. helveticus KLDS1.8701 is safe and can be used as a potential probiotic for human consumption. View Full-Text
Keywords: probiotics; Lactobacillus helveticus; safety assessment; genomics; oral toxicity study; cecal microbiota probiotics; Lactobacillus helveticus; safety assessment; genomics; oral toxicity study; cecal microbiota
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Li, B.; Jin, D.; Etareri Evivie, S.; Li, N.; Yan, F.; Zhao, L.; Liu, F.; Huo, G. Safety Assessment of Lactobacillus helveticus KLDS1.8701 Based on Whole Genome Sequencing and Oral Toxicity Studies. Toxins 2017, 9, 301.

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