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Toxins 2013, 5(10), 1859-1871;

Assessment of the Functional Regions of the Superantigen Staphylococcal Enterotoxin B

Center for Inflammation, Translational and Clinical Lung Research, Temple University School of Medicine, 3500 N. Broad Street, Philadelphia, PA 19140, USA
Author to whom correspondence should be addressed.
Received: 8 August 2013 / Revised: 9 October 2013 / Accepted: 10 October 2013 / Published: 22 October 2013
(This article belongs to the Special Issue Enterotoxins: Microbial Proteins and Host Cell Dysregulation)
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The functional activity of superantigens is based on capacity of these microbial proteins to bind to both the β-chain of the T cell receptor (TcR) and the major histocompatibility complex (MHC) class II dimer. We have previously shown that a subset of the bacterial superantigens also binds to a membrane protein, designated p85, which is expressed by renal epithelial cells. This binding activity is a property of SEB, SEC1, 2 and 3, but not SEA, SED, SEE or TSST. The crystal structure of the tri-molecular complex of the superantigen staphylococcal enterotoxin B (SEB) with both the TcR and class II has previously been reported. However, the relative contributions of regions of the superantigen to the overall functional activity of this superantigen remain undefined. In an effort to better define the molecular basis for the interaction of SEB with the TcR β-chain, we report studies here which show the comparative contributions of amino- and carboxy-terminal regions in the superantigen activity of SEB. Recombinant fusion proteins composed of bacterial maltose-binding protein linked to either full-length or truncated toxins in which the 81 N-terminal, or 19 or 34 C-terminal amino acids were deleted, were generated for these studies. This approach provides a determination of the relative strength of the functional activity of the various regions of the superantigen protein. View Full-Text
Keywords: superantigen; staphylococcal enterotoxin B; MHC class II; T cell receptor superantigen; staphylococcal enterotoxin B; MHC class II; T cell receptor

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Zhang, L.; Rogers, T.J. Assessment of the Functional Regions of the Superantigen Staphylococcal Enterotoxin B. Toxins 2013, 5, 1859-1871.

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