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Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer

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Southern Regional Research Center, ARS/USDA, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA
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Department of Biology, 1 Drexel Dr., Box 85B, Xavier University of Louisiana, New Orleans, LA 70125, USA
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Author to whom correspondence should be addressed.
Toxins 2011, 3(8), 932-948; https://doi.org/10.3390/toxins3080932
Received: 25 May 2011 / Revised: 19 July 2011 / Accepted: 26 July 2011 / Published: 2 August 2011
(This article belongs to the Special Issue Aflatoxins 2011)
Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce aflatoxins. To understand how these treatments affect expression of genes involved in aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify genes in treated clones that are differentially expressed compared to the wild-type. Expression of 183 genes was significantly dysregulated. Of these, 38 had at least two-fold or lower expression compared to the untreated control and only two had two-fold or higher expression. The most frequent change was downregulation of genes predicted to encode membrane-bound proteins. Based on this result we hypothesize that the treatments cause changes in the structure of cellular and organelle membranes that prevent normal development and aflatoxin biosynthesis. View Full-Text
Keywords: aflatoxin; microarray; 5-azacytidine; Aspergillus parasiticus; secondary metabolism; fluffy phenotype aflatoxin; microarray; 5-azacytidine; Aspergillus parasiticus; secondary metabolism; fluffy phenotype
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Wilkinson, J.R.; Kale, S.P.; Bhatnagar, D.; Yu, J.; Ehrlich, K.C. Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer. Toxins 2011, 3, 932-948.

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