Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Isolation of Human RBCs
2.3. Preparation of LtxA and LtxA-FITC
2.4. Ganglioside Blocking Assays
2.5. Flow Cytometry
2.6. Cytotoxicity Assays
2.7. Statistical Analysis
3. Results
3.1. LtxA Does Not Use Glycophorin as a Receptor
3.2. Gangliosides Block LtxA-Mediated Hemolysis
Compound | Blocking |
---|---|
α-lactose | - |
D (-) fructose | - |
D (+) galactose | - |
D-glucose | - |
D-maltose | - |
D-arabinose | - |
D-ribose | - |
Sucrose | - |
D (+) mannose | - |
D (+) xylose | - |
N-acetylmuramic acid | - |
Ganglioside GM1 | + |
Ganglioside GD1b | + |
3.3. LtxA Binds to Gangliosides on Glioma Cells
3.4. Gangliosides Inefficiently Block LtxA-Mediated Killing of WBCs
4. Discussion
Acknowledgements
References
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Forman, M.S.; Nishikubo, J.B.; Han, R.K.; Le, A.; Balashova, N.V.; Kachlany, S.C. Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis. Toxins 2010, 2, 2824-2836. https://doi.org/10.3390/toxins2122824
Forman MS, Nishikubo JB, Han RK, Le A, Balashova NV, Kachlany SC. Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis. Toxins. 2010; 2(12):2824-2836. https://doi.org/10.3390/toxins2122824
Chicago/Turabian StyleForman, Michael S., Jason B. Nishikubo, Rebecca K. Han, Amy Le, Nataliya V. Balashova, and Scott C. Kachlany. 2010. "Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis" Toxins 2, no. 12: 2824-2836. https://doi.org/10.3390/toxins2122824
APA StyleForman, M. S., Nishikubo, J. B., Han, R. K., Le, A., Balashova, N. V., & Kachlany, S. C. (2010). Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis. Toxins, 2(12), 2824-2836. https://doi.org/10.3390/toxins2122824