Next Article in Journal / Special Issue
Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying
Previous Article in Journal / Special Issue
Image-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions
Open AccessCommunication

Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier

Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Keiji Itaka
Pharmaceutics 2015, 7(3), 224-232; https://doi.org/10.3390/pharmaceutics7030224
Received: 30 May 2015 / Revised: 5 August 2015 / Accepted: 20 August 2015 / Published: 25 August 2015
(This article belongs to the Special Issue New Paradigm of Gene Therapy)
Methylated poly(l-histidine) (PLH-Me), our original polypeptide, has controlled the contents of dimethylimidazolium, τ/π-methylimidazole and imidazole groups for efficient gene delivery. The screening for the PLH-Me as DNA carrier has been carried out by use of the PLH with 25 mol% (τ-methyl, 16 mol%; π-methyl, 17 mol%; deprotonated imidazole, 41 mol%), 68 mol% (τ-methyl, 16 mol%; π-methyl, 8 mol%; deprotonated imidazole, 8 mol%) and 87 mol% (τ-methyl, 7 mol%; π-methyl, 4 mol%; deprotonated imidazole, 2 mol%) dimethylimidazolium groups, that is, PLH-Me(25), PLH-Me(68) and PLH-Me(87), respectively. The screening of the chemical structure of PLH-Me has been carried out for DNA carrier properties, which are the stability of its DNA polyion complexes and gene expression. The DNA complexes with the 25 mol% and 68 mol% dimethylated PLH-Me possessed almost same ability to retain DNA, as compared with the 87 mol% dimethylated PLH-Me, which was examined by competitive exchange with dextran sulfate. From the gene transfection experiment against HepG2 cells, human hepatoma cell line, the PLH-Me(25)/DNA complex was revealed to mediate highest gene expression. These results suggest that the dimethyl-imidazolium/methylimidazole/imidazole balance of the PLH-Me is important for DNA carrier design. View Full-Text
Keywords: methylated poly(l-histidine); DNA carrier; dimethylimidazolium group; τ/π-methylimidazole group; imidazole group methylated poly(l-histidine); DNA carrier; dimethylimidazolium group; τ/π-methylimidazole group; imidazole group
Show Figures

Graphical abstract

MDPI and ACS Style

Asayama, S.; Kumagai, T.; Kawakami, H. Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier. Pharmaceutics 2015, 7, 224-232. https://doi.org/10.3390/pharmaceutics7030224

AMA Style

Asayama S, Kumagai T, Kawakami H. Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier. Pharmaceutics. 2015; 7(3):224-232. https://doi.org/10.3390/pharmaceutics7030224

Chicago/Turabian Style

Asayama, Shoichiro; Kumagai, Takao; Kawakami, Hiroyoshi. 2015. "Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier" Pharmaceutics 7, no. 3: 224-232. https://doi.org/10.3390/pharmaceutics7030224

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop