A Comparative Study of Transmembrane Diffusion and Permeation of Ibuprofen across Synthetic Membranes Using Franz Diffusion Cells
Abstract
:1. Introduction
2. Experimental Section
2.1. Synthetic membranes
Membrane | Polymera | MWCO (kDa) | Pore size (µm) | Thickness(µm) | ρ (%) | τ | Source | Batch no. |
---|---|---|---|---|---|---|---|---|
Cellulose-based | ||||||||
Visking | RC | 12-14 | - | 20b | - | - | Medicell (London, UK) | DTV12000.05.000 |
Cuprophan | RC | 10 | - | 10b | - | - | Medicell (London, UK) | N/A |
Benzoylated tubing | RC | 1.2-2 | - | 35b | - | - | Sigma (Dorset,UK) | 074K7012 |
Cellulose ester | CE | 0.5 | - | 80b | - | - | Spectrumlab (California) | 131060 |
Cellulose nitrate | CN | 0.45 | 125 | 66-84 | - | Whatman (UK) | N/A | |
Polymeric-based | ||||||||
AN 69 | PAN | 40 | - | 25b | - | - | Hospal (Huntingdon, UK) | N/A |
Biodyne | PA | na | 0.45 | 152 | 50- | - | Pall (Portsmouth, UK) | b- 50046, c-189051 |
Supor | PES | na | 0.45 | 145 | 75 | ~1-1.5 | Pall (Portsmouth, UK) | 55083 |
Tuffryn | PS | na | 0.45 | 145 | 80 | ~1-1.5 | Pall (Portsmouth, UK) | 60669 |
Nuclepore | PC | na | 0.1 | 10 | 60 | ~1 | Whatman (New Jersey, USA) | 6018023 |
Cyclopore | PC | na | 0.1 | 10 | 8 | ~1 | Whatman (New Jersey, USA) | 060.0131/6E8/L-3-L |
Celgard 3500 | PP | na | 0.05 | 20 | 4 | - | Hoechst (New Jersey, USA) | 293485 |
Silicone | PDMS | na | - | 400 | 35-48 | - | SAMCO (Nuneaton, UK) | 19T0.3-1000-60M1 |
2.2. Franz diffusion cells apparatus
2.3. Other materials
2.4. Preparation of ibuprofen saturated solution
2.5. Ibuprofen Saturated solubility
2.6. Preparation of ibuprofen crystals
2.7. Membrane treatment
2.8. Franz diffusion cell studies
2.8.1. Effect of synthetic membranes on ibuprofen drug diffusion
2.8.2. Comparison of ibuprofen drug diffusion through membrane of different pore sizes and surface groups
2.8.3. Regression analysis of ibuprofen flux with membrane parameters
2.9. Statistical analysis
3. Results and Discussion
3.1. Drug solubility and mass balance
Preparation # | Amount of ibuprofen added (g) | Amount remaining on the filter paper (g) | Amout of ibuprofen dissolved in 50 mL (g) | Concentration% (w/v) |
---|---|---|---|---|
1 | 1.657 | 0.616 | 1.041 | 2.082 |
2 | 1.621 | 0.591 | 1.030 | 2.060 |
3 | 1.637 | 0.579 | 1.058 | 2.116 |
4 | 1.620 | 0.559 | 1.061 | 2.122 |
5 | 1.767 | 0.746 | 1.021 | 2.042 |
3.2. Ibuprofen drug diffusion across synthetic membranes
Membrane | Flux (mg/cm2/h) | Total diffusion after 6 h (mg/cm2) | CV (%) |
---|---|---|---|
Cellulose nitrate | 17.65 ± 2.06 | 97.89 ± 5.79 | 11.7 |
Nuclepore | 17.38 ± 0.79 | 97.75 ± 4.01 | 4.6 |
Celgard | 15.45 ± 1.02 | 67.28 ± 3.66 | 6.6 |
Cyclopore | 14.87 ± 0.50 | 86.32 ± 2.77 | 3.4 |
Tuffryn | 13.54 ± 0.49 | 77.23 ± 4.80 | 3.6 |
Supor | 10.48 ± 0.31 | 63.63 ± 2.08 | 2.9 |
AN69 | 8.14 ± 0.38 | 47.84 ± 2.25 | 4.7 |
*Cellulose ester | 2.66 ± 0.19 | 16.30 ± 0.84 | 7.3 |
Biodyne B | 1.96 ± 0.07 | 19.32 ± 0.62 | 3.6 |
Biodyne C | 1.77 ± 0.17 | 18.44 ± 0.59 | 4.4 |
Cuprophan | 1.57 ± 0.15 | 17.94 ± 0.69 | 4.7 |
Benzoylated cellulose | 1.51 ± 0.04 | 13.32 ± 0.24 | 2.9 |
Visking | 1.39 ± 0.09 | 9.88 ± 0.37 | 6.2 |
PDMS | 0.09 ± 0.01 | 0.45 ± 0.002 | 6.0 |
3.3. Correlation of flux with membrane parameters
3.4. Discussion
4. Conclusions
Acknowledgements
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Ng, S.-F.; Rouse, J.; Sanderson, D.; Eccleston, G. A Comparative Study of Transmembrane Diffusion and Permeation of Ibuprofen across Synthetic Membranes Using Franz Diffusion Cells. Pharmaceutics 2010, 2, 209-223. https://doi.org/10.3390/pharmaceutics2020209
Ng S-F, Rouse J, Sanderson D, Eccleston G. A Comparative Study of Transmembrane Diffusion and Permeation of Ibuprofen across Synthetic Membranes Using Franz Diffusion Cells. Pharmaceutics. 2010; 2(2):209-223. https://doi.org/10.3390/pharmaceutics2020209
Chicago/Turabian StyleNg, Shiow-Fern, Jennifer Rouse, Dominic Sanderson, and Gillian Eccleston. 2010. "A Comparative Study of Transmembrane Diffusion and Permeation of Ibuprofen across Synthetic Membranes Using Franz Diffusion Cells" Pharmaceutics 2, no. 2: 209-223. https://doi.org/10.3390/pharmaceutics2020209