Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Culture
2.2. Cell Cytotoxicity
2.3. Migration and Invasion Assay
2.4. CS2-Empty Vector and CS2-S100A4 Transfection
2.5. Western Blot Assay
2.6. Real-Time RT-PCR
2.7. Statistical Analyses
3. Results
3.1. Effects of Arctiin on the Viability of Cervical Cancer Cells
3.2. Inhibitory Effects of Arctiin on Cell Migration and Invasion in Cervical Cancer Cells
3.3. Effects of Arctiin on the S100A4 Protein and Mrna Level
3.4. Effects of Arctiin on PI3K/Akt Pathway in Cervical Cancer Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, C.-Y.; Hsin, M.-C.; Chen, P.-N.; Lin, C.-W.; Wang, P.-H.; Yang, S.-F.; Hsiao, Y.-H. Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway. Pharmaceutics 2022, 14, 365. https://doi.org/10.3390/pharmaceutics14020365
Lee C-Y, Hsin M-C, Chen P-N, Lin C-W, Wang P-H, Yang S-F, Hsiao Y-H. Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway. Pharmaceutics. 2022; 14(2):365. https://doi.org/10.3390/pharmaceutics14020365
Chicago/Turabian StyleLee, Chung-Yuan, Min-Chieh Hsin, Pei-Ni Chen, Chiao-Wen Lin, Po-Hui Wang, Shun-Fa Yang, and Yi-Hsuan Hsiao. 2022. "Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway" Pharmaceutics 14, no. 2: 365. https://doi.org/10.3390/pharmaceutics14020365
APA StyleLee, C.-Y., Hsin, M.-C., Chen, P.-N., Lin, C.-W., Wang, P.-H., Yang, S.-F., & Hsiao, Y.-H. (2022). Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway. Pharmaceutics, 14(2), 365. https://doi.org/10.3390/pharmaceutics14020365