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Article

Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis

1
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
2
Department of Microbiology and Immunology, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, Cairo 12573, Egypt
3
Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
4
Department of Clinical Pathology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
5
School of Pharmacy, Newgiza University, Giza 12588, Egypt
*
Author to whom correspondence should be addressed.
Academic Editors: Hyun-ouk Kim and Yogeshvar N. Kalia
Pharmaceutics 2021, 13(8), 1141; https://doi.org/10.3390/pharmaceutics13081141
Received: 30 May 2021 / Revised: 9 July 2021 / Accepted: 22 July 2021 / Published: 26 July 2021
Neonatal sepsis is a leading cause of death among newborns and infants, especially in the developing world. The problem is compounded by the delays in pinpointing the causative agent of the infection. This is reflected in increasing mortality associated with these cases and the spread of multi-drug-resistant bacteria. In this work, we deployed bioinformatics and proteomics analyses to determine a promising target that could be used for the identification of a major neonatal sepsis causative agent, Klebsiella pneumoniae. A 19 amino acid peptide from a hypothetical outer membrane was found to be very specific to the species, well conserved among its strains, surface exposed, and expressed in conditions simulating infection. Antibodies against the selected peptide were conjugated to gold nanoparticles and incorporated into an immunochromatographic strip. The developed strip was able to detect as low as 105 CFU/mL of K. pneumoniae. Regarding specificity, it showed negative results with both Escherichia coli and Enterobacter cloacae. More importantly, in a pilot study using neonatal sepsis cases blood specimens, the developed strip selectively gave positive results within 20 min with those infected with K. pneumoniae without prior sample processing. However, it gave negative results in cases infected with other bacterial species. View Full-Text
Keywords: K. pneumoniae; neonatal sepsis; immunochromatographic strip; diagnosis; rapid; gold nanoparticles K. pneumoniae; neonatal sepsis; immunochromatographic strip; diagnosis; rapid; gold nanoparticles
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MDPI and ACS Style

Elhosseiny, N.M.; Samir, T.M.; Ali, A.A.; El-Kholy, A.A.; Attia, A.S. Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis. Pharmaceutics 2021, 13, 1141. https://doi.org/10.3390/pharmaceutics13081141

AMA Style

Elhosseiny NM, Samir TM, Ali AA, El-Kholy AA, Attia AS. Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis. Pharmaceutics. 2021; 13(8):1141. https://doi.org/10.3390/pharmaceutics13081141

Chicago/Turabian Style

Elhosseiny, Noha M., Tamer M. Samir, Aliaa A. Ali, Amani A. El-Kholy, and Ahmed S. Attia 2021. "Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis" Pharmaceutics 13, no. 8: 1141. https://doi.org/10.3390/pharmaceutics13081141

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