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Endocytic Uptake of Solid Lipid Nanoparticles by the Nasal Mucosa

Department of Pharmaceutical Science and Experimental Therapeutics, College of Pharmacy, University of Iowa, 115 S. Grand Avenue, Pharmacy Building, Iowa City, IA 52242, USA
Author to whom correspondence should be addressed.
Academic Editor: Eryvaldo Sócrates Tabosa Egito
Pharmaceutics 2021, 13(5), 761;
Received: 29 April 2021 / Revised: 13 May 2021 / Accepted: 17 May 2021 / Published: 20 May 2021
(This article belongs to the Special Issue Lipid-Based Dispersed Systems for Drug Delivery)
Nanoparticles may provide unique therapeutic opportunities when administered via the nasal cavity, yet the primary uptake and transfer pathways for these particles within the nasal mucosa are not well understood. The endocytic pathways involved in the uptake of fluorescently labeled, (Nile Red) solid lipid nanoparticles (SLNs) of different sizes (~30, 60, and 150 nm) were studied using excised bovine olfactory and nasal respiratory tissues. Endocytic activity contributing to nanoparticle uptake was investigated using a variety of pharmacological inhibitors, but none of the inhibitors were able to completely eliminate the uptake of the SLNs. The continued uptake of nanoparticles following exposure to individual inhibitors suggests that a number of endocytic pathways work in combination to transfer nanoparticles into the nasal mucosa. Following exposure to the general metabolic inhibitors, 2,4-DNP and sodium azide, additional, non-energy-dependent pathways for nanoparticle uptake were also observed. While the smallest nanoparticles (30 nm) were the most resistant to the effects of pharmacologic inhibitors, the largest (150 nm) were still able to transfer significant amounts of the particles into the tissues. The rapid nanoparticle uptake observed demonstrates that these lipid particles are promising vehicles to accomplish both local and systemic drug delivery following nasal administration. View Full-Text
Keywords: solid lipid nanoparticles; endocytosis; intranasal drug delivery solid lipid nanoparticles; endocytosis; intranasal drug delivery
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MDPI and ACS Style

Al Khafaji, A.S.; Donovan, M.D. Endocytic Uptake of Solid Lipid Nanoparticles by the Nasal Mucosa. Pharmaceutics 2021, 13, 761.

AMA Style

Al Khafaji AS, Donovan MD. Endocytic Uptake of Solid Lipid Nanoparticles by the Nasal Mucosa. Pharmaceutics. 2021; 13(5):761.

Chicago/Turabian Style

Al Khafaji, Ammar S., and Maureen D. Donovan 2021. "Endocytic Uptake of Solid Lipid Nanoparticles by the Nasal Mucosa" Pharmaceutics 13, no. 5: 761.

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