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Pharmaceutics
Volume 12
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10.3390/pharmaceutics12030224
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Article

Doxorubicin–Loaded Human Serum Albumin Submicron Particles: Preparation, Characterization and In Vitro Cellular Uptake

by
Saranya Chaiwaree
1,2,
Ausanai Prapan
1,3,
Nittiya Suwannasom
1,4,
Tomás Laporte
1,5,
Tanja Neumann
6,
Axel Pruß
1,
Radostina Georgieva
1,7 and
Hans Bäumler
1,*
1
Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany
2
Department of Pharmaceutical Technology, Faculty of Pharmacy, Payap University, Chiang Mai 50000, Thailand
3
Department of Radiological Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand
4
Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
5
Instituto de Nanosistemas, Universidad Nacional de San Martín, San Martín, Pcia de Buenos Aires 1021, Argentina
6
JPK BioAFM Business, Nano Surfaces Division, Bruker Nano GmbH, 12489 Berlin, Germany
7
Department of Medical Physics, Biophysics and Radiology, Medical Faculty, Trakia University, 6000 Stara Zagora, Bulgaria
*
Author to whom correspondence should be addressed.
Pharmaceutics 2020, 12(3), 224; https://doi.org/10.3390/pharmaceutics12030224
Submission received: 11 February 2020 / Revised: 28 February 2020 / Accepted: 28 February 2020 / Published: 2 March 2020
(This article belongs to the Section Drug Delivery and Controlled Release)
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Abstract

Doxorubicin (DOX) is an effective anthracycline antibiotic drug which is commonly used in a broad range cancer therapy. However, due to dose depending side effects and toxicity to non-cancerous tissues, its clinical applications are restricted. To overcome these limitations, human serum albumin (HSA) has been investigated as a biocompatible drug delivery vehicle. In this study, human serum albumin submicron particles (HSA-MPs) were fabricated by using the Co-precipitation–Crosslinking–Dissolution technique (CCD technique) and DOX was loaded into the protein particles by absorption. DOX-HSA-MPs showed uniform peanut-like shape, submicron size and negative zeta-potential (−13 mV). The DOX entrapment efficiency was 25% of the initial amount. The in vitro release in phosphate buffered saline pH 7.4 was less than 1% within 5 h. In contrast, up to 40% of the entrapped DOX was released in presence of a protein digesting enzyme mixture (Pronase®) within the same time. In addition, in vitro cytotoxicity and cellular uptake of DOX-HSA-MPs were evaluated using the lung carcinoma cell line A549. The results demonstrated that DOX-HSA-MPs reduced the cell metabolic activities after 72 h. Interestingly, DOX-HSA-MPs were taken up by A549 cells up to 98% and localized in the cell lysosomal compartment. This study suggests that DOX-HSA-MPs which was fabricated by CCD technique is seen as a promising biopolymer particle as well as a viable alternative for drug delivery application to use for cancer therapy.
Keywords: doxorubicin; albumin particles; CCD technique; cellular uptake; submicron particles doxorubicin; albumin particles; CCD technique; cellular uptake; submicron particles
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MDPI and ACS Style

Chaiwaree, S.; Prapan, A.; Suwannasom, N.; Laporte, T.; Neumann, T.; Pruß, A.; Georgieva, R.; Bäumler, H. Doxorubicin–Loaded Human Serum Albumin Submicron Particles: Preparation, Characterization and In Vitro Cellular Uptake. Pharmaceutics 2020, 12, 224. https://doi.org/10.3390/pharmaceutics12030224

AMA Style

Chaiwaree S, Prapan A, Suwannasom N, Laporte T, Neumann T, Pruß A, Georgieva R, Bäumler H. Doxorubicin–Loaded Human Serum Albumin Submicron Particles: Preparation, Characterization and In Vitro Cellular Uptake. Pharmaceutics. 2020; 12(3):224. https://doi.org/10.3390/pharmaceutics12030224

Chicago/Turabian Style

Chaiwaree, Saranya, Ausanai Prapan, Nittiya Suwannasom, Tomás Laporte, Tanja Neumann, Axel Pruß, Radostina Georgieva, and Hans Bäumler. 2020. "Doxorubicin–Loaded Human Serum Albumin Submicron Particles: Preparation, Characterization and In Vitro Cellular Uptake" Pharmaceutics 12, no. 3: 224. https://doi.org/10.3390/pharmaceutics12030224

APA Style

Chaiwaree, S., Prapan, A., Suwannasom, N., Laporte, T., Neumann, T., Pruß, A., Georgieva, R., & Bäumler, H. (2020). Doxorubicin–Loaded Human Serum Albumin Submicron Particles: Preparation, Characterization and In Vitro Cellular Uptake. Pharmaceutics, 12(3), 224. https://doi.org/10.3390/pharmaceutics12030224

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