In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein Design and Production
2.2. Protein Purification
2.3. Nanoparticle Size Characterization
2.4. Bactericidal Activity Assay
2.5. Disassembling of Soluble Nanoparticles
2.6. Protein Solubilization from IBs
2.7. Determination of Murine IFN-γ Biological Activity
2.8. Mouse Model of Infectious Mastitis
2.9. Statistical Analysis
3. Results
3.1. Characterization of Self-Assembling Nanoparticles
3.2. In Vitro Evaluation of Antibacterial Activity
3.3. Importance of Nanoparticle Format on Antibacterial Activity
3.4. Bioactivity Evaluation of IFN-γ-Based Proteins
3.5. Growth Kinetics of E. coli in the Mastitis Mice Model
3.6. Characterization of IBs
3.7. Therapeutic Effect of PNPs and IBs in the Mouse Mastitis Model
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Carratalá, J.V.; Brouillette, E.; Serna, N.; Sánchez-Chardi, A.; Sánchez, J.M.; Villaverde, A.; Arís, A.; Garcia-Fruitós, E.; Ferrer-Miralles, N.; Malouin, F. In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics. Pharmaceutics 2020, 12, 1217. https://doi.org/10.3390/pharmaceutics12121217
Carratalá JV, Brouillette E, Serna N, Sánchez-Chardi A, Sánchez JM, Villaverde A, Arís A, Garcia-Fruitós E, Ferrer-Miralles N, Malouin F. In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics. Pharmaceutics. 2020; 12(12):1217. https://doi.org/10.3390/pharmaceutics12121217
Chicago/Turabian StyleCarratalá, Jose V., Eric Brouillette, Naroa Serna, Alejandro Sánchez-Chardi, Julieta M. Sánchez, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Neus Ferrer-Miralles, and François Malouin. 2020. "In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics" Pharmaceutics 12, no. 12: 1217. https://doi.org/10.3390/pharmaceutics12121217