Physicochemical Properties and Hematocompatibility of Layered Double Hydroxide-Based Anticancer Drug Methotrexate Delivery System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Pristine LDH and MTX-LDH
2.3. Characterization of MTX-LDH
2.4. In Vitro Biological Assay: Plasma Protein Fluorescence Quenching
2.5. In Vivo Biological Assay: Hematocompatibility in Mouse Model
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Protein | Sample Name | n | KD | Kb | r2 |
---|---|---|---|---|---|
Albumin | MTX-LDH | 3.83 | 2.20 × 10−1 | 4.54 | 0.935 |
MTX | 2.04 | 1.56 × 10−2 | 6.42 × 101 | 0.988 | |
LDH | 1.39 | 4.5 × 10−1 | 2.21 | 0.968 | |
Fibrinogen | MTX-LDH | 3.87 | 2.59 × 10−1 | 3.87 | 0.911 |
MTX | 8.50 × 10−1 | 6.68 × 10−3 | 1.50 × 102 | 0.923 | |
LDH | 1.47 | 4.19 × 10−1 | 2.39 | 0.928 | |
Globulin | MTX-LDH | 3.89 | 2.63 × 10−1 | 3.8 | 0.971 |
MTX | 2.45 | 2.17 × 10−2 | 4.62 × 101 | 0.953 | |
LDH | 1.39 | 4.45 × 10−1 | 2.25 | 0.939 |
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Jung, S.-Y.; Kim, H.-M.; Hwang, S.; Jeung, D.-G.; Rhee, K.-J.; Oh, J.-M. Physicochemical Properties and Hematocompatibility of Layered Double Hydroxide-Based Anticancer Drug Methotrexate Delivery System. Pharmaceutics 2020, 12, 1210. https://doi.org/10.3390/pharmaceutics12121210
Jung S-Y, Kim H-M, Hwang S, Jeung D-G, Rhee K-J, Oh J-M. Physicochemical Properties and Hematocompatibility of Layered Double Hydroxide-Based Anticancer Drug Methotrexate Delivery System. Pharmaceutics. 2020; 12(12):1210. https://doi.org/10.3390/pharmaceutics12121210
Chicago/Turabian StyleJung, Sang-Yong, Hyoung-Mi Kim, Soonjae Hwang, Do-Gak Jeung, Ki-Jong Rhee, and Jae-Min Oh. 2020. "Physicochemical Properties and Hematocompatibility of Layered Double Hydroxide-Based Anticancer Drug Methotrexate Delivery System" Pharmaceutics 12, no. 12: 1210. https://doi.org/10.3390/pharmaceutics12121210