Immunoregulatory and Antimicrobial Activity of Bovine Neutrophil β-Defensin-5-Loaded PLGA Nanoparticles against Mycobacterium bovis
Abstract
:1. Introduction
2. Methods
2.1. Mice
2.2. Bacterial Culture
2.3. Preparation of B5-NPs
2.4. Determination of B5-Encapsulation and -Loading Efficiency
2.5. In Vitro Release Kinetics of B5-NPs
2.6. Cell Culture and Stimulation
2.7. Mice Immunization and M. bovis Challenge
2.8. Enzyme-Linked Immunosorbent Assay (ELISA) Assay
2.9. Flow Cytometry
2.10. Colony-Forming Units (CFU) Enumeration
2.11. Lung Histology
2.12. Statistical Analysis
3. Results
3.1. Physicochemical and Morphological Characterization of B5-NPs
3.2. B5-NPs Enhance the Cytokine Secretion in J774A.1 Cells
3.3. B5-NPs Promote TNF-α and IgA Production in Mice
3.4. B5-NPs Increase the Population of CD4+ and CD8+ T Cells in Spleen
3.5. B5-NPs Enhance Protective Efficacy against M. bovis
3.6. B5 Promotes the Therapeutic Effect of RIF against M. bovis
3.7. B5 and B5-NPs Reduce M. bovis-Induced TNF-α Hypersecretion
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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PLGA-NPs | Size (nm) | PDI | Zeta Potential (mV) | EE (%) | LE (%) |
---|---|---|---|---|---|
PBS-NPs | 193.2 ± 22.0 | 0.16 ± 0.03 | −25.1 ± 1.5 | ||
B5-NPs | 206.6 ± 26.6 | 0.16 ± 0.04 | −27.1 ± 1.5 | 85.5 ± 2.5 | 0.5 ± 0.02 |
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Liang, Z.; Liu, Y.; Sun, X.; Lin, J.; Yao, J.; Song, Y.; Li, M.; Liu, T.; Zhou, X. Immunoregulatory and Antimicrobial Activity of Bovine Neutrophil β-Defensin-5-Loaded PLGA Nanoparticles against Mycobacterium bovis. Pharmaceutics 2020, 12, 1172. https://doi.org/10.3390/pharmaceutics12121172
Liang Z, Liu Y, Sun X, Lin J, Yao J, Song Y, Li M, Liu T, Zhou X. Immunoregulatory and Antimicrobial Activity of Bovine Neutrophil β-Defensin-5-Loaded PLGA Nanoparticles against Mycobacterium bovis. Pharmaceutics. 2020; 12(12):1172. https://doi.org/10.3390/pharmaceutics12121172
Chicago/Turabian StyleLiang, Zhengmin, Yiduo Liu, Xingya Sun, Jingjun Lin, Jiao Yao, Yinjuan Song, Miaoxuan Li, Tianlong Liu, and Xiangmei Zhou. 2020. "Immunoregulatory and Antimicrobial Activity of Bovine Neutrophil β-Defensin-5-Loaded PLGA Nanoparticles against Mycobacterium bovis" Pharmaceutics 12, no. 12: 1172. https://doi.org/10.3390/pharmaceutics12121172