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Open AccessArticle

Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

1
Children’s Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, NSW 2031, Australia
2
Department of Pharmacy Health and Nutritional Science, University of Calabria, 87036 Rende (CS), Italy
3
nB nanoSacale Biomagnetics SL, 50012 Zaragoza, Spain
4
ARC Centre of Excellence for Convergent BioNano Science and Technology, Australian Centre for NanoMedicine, UNSW Sydney, NSW 2052, Australia
5
School of Women’s and Children’s Health, Faculty of Medicine, UNSW Sydney, NSW 2052, Australia
6
OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
7
German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
8
German Cancer Consortium (DKTK), partner site Dresden, 01307 Dresden, Germany
9
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiooncology-Oncoray, 01307 Dresden, Germany
10
Leibniz Institute of Solid State and Material Research Dresden, 01069 Dresden, Germany
11
Institute of Nanoscience of Aragon (INA), Department of Condensed Matter Physics, University of Zaragoza, 50018 Zaragoza, Spain
*
Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(1), 3; https://doi.org/10.3390/pharmaceutics11010003
Received: 27 November 2018 / Revised: 14 December 2018 / Accepted: 18 December 2018 / Published: 22 December 2018
With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the [email protected] system and albumin bioconjugate, consisted of [email protected] nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site. View Full-Text
Keywords: graphene oxide; iron oxide nanoparticles; magnetic targeting; nanohybrids; synergism graphene oxide; iron oxide nanoparticles; magnetic targeting; nanohybrids; synergism
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MDPI and ACS Style

Lerra, L.; Farfalla, A.; Sanz, B.; Cirillo, G.; Vittorio, O.; Voli, F.; Le Grand, M.; Curcio, M.; Nicoletta, F.P.; Dubrovska, A.; Hampel, S.; Iemma, F.; Goya, G.F. Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells. Pharmaceutics 2019, 11, 3.

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