Perturbation of Human T-Cell Leukemia Virus Type 1 Particle Morphology by Differential Gag Co-Packaging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production and Purification of HTLV-1-Like Particles
2.2. Cryogenic Transmission Electron Microscopy Analysis of HTLV-1-Like Particles
2.3. Measurement of Virus-Like Particle Size
2.4. Fluorescence Fluctuation Spectroscopy, Experimental Setup, and Data Analysis
2.5. Scanning Transmission Electron Microscopy Mass Measurements of Virus-Like Particles
2.6. Protein and RNA Content of HTLV-1 Virus-Like Particles
3. Results
3.1. Morphology of HTLV-1-Like Particles
3.2. Gag Stoichiometry of HTLV-1-Like Particles Determined by Fluorescence Fluctuation Spectroscopy
3.3. Virus-Like Particles Diameter as Determined by FFS
3.4. Scanning Transmission Electro Microscopy Analysis of HTLV-1-Like Particle Mass
3.5. Scanning Transmission Electron Microscopy Determination of Gag Stoichiometry in HTLV-1-Like Particles
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Measurement | HTLV-1 Like Particle | ||
---|---|---|---|
Gag-YFP | Gag:Gag-YFP (3:1) | ||
Average Diameter (nm) a | 83 | 113 | |
Average Particle Mass (MDa) b | 80 | 219 | |
Mass of RNA, Lipid and Protein (MDa) | RNA c | 2.4 | 6.6 |
Lipid d | 36 | 80 | |
Total protein e | 42 | 133 | |
Mass of Gag Molecules (MDa) | Total Gag polyprotein f | 29–38 | 93–120 |
Gag | - | 70–90 | |
Gag-YFP | 29–38 | 23–30 | |
Gag polyprotein copy number g | 360–480 | 1600–2100 |
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Maldonado, J.O.; Angert, I.; Cao, S.; Berk, S.; Zhang, W.; Mueller, J.D.; Mansky, L.M. Perturbation of Human T-Cell Leukemia Virus Type 1 Particle Morphology by Differential Gag Co-Packaging. Viruses 2017, 9, 191. https://doi.org/10.3390/v9070191
Maldonado JO, Angert I, Cao S, Berk S, Zhang W, Mueller JD, Mansky LM. Perturbation of Human T-Cell Leukemia Virus Type 1 Particle Morphology by Differential Gag Co-Packaging. Viruses. 2017; 9(7):191. https://doi.org/10.3390/v9070191
Chicago/Turabian StyleMaldonado, José O., Isaac Angert, Sheng Cao, Serkan Berk, Wei Zhang, Joachim D. Mueller, and Louis M. Mansky. 2017. "Perturbation of Human T-Cell Leukemia Virus Type 1 Particle Morphology by Differential Gag Co-Packaging" Viruses 9, no. 7: 191. https://doi.org/10.3390/v9070191