Next Article in Journal
Characterization and Temperature Dependence of Arctic Micromonas polaris Viruses
Next Article in Special Issue
Mechanisms of Adaptive Immunity to Porcine Reproductive and Respiratory Syndrome Virus
Previous Article in Journal
Epigenetic Control of Human Endogenous Retrovirus Expression: Focus on Regulation of Long-Terminal Repeats (LTRs)
Previous Article in Special Issue
UK Pigs at the Time of Slaughter: Investigation into the Correlation of Infection with PRRSV and HEV
Open AccessArticle

Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site

1
Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain
2
Department of Infectious Diseases, King’s College London School of Medicine, Guy’s Hospital, London SE1 9RT, UK
3
Department of Microbiology and Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
*
Author to whom correspondence should be addressed.
Viruses 2017, 9(6), 133; https://doi.org/10.3390/v9060133
Received: 4 April 2017 / Revised: 19 May 2017 / Accepted: 25 May 2017 / Published: 1 June 2017
(This article belongs to the Special Issue Porcine Viruses)
African swine fever virus (ASFV) infection causes endosomal reorganization. Here, we show that the virus causes endosomal congregation close to the nucleus as the infection progresses, which is necessary to build a compact viral replication organelle. ASFV enters the cell by the endosomal pathway and reaches multivesicular late endosomes. Upon uncoating and fusion, the virus should exit to the cytosol to start replication. ASFV remodels endosomal traffic and redistributes endosomal membranes to the viral replication site. Virus replication also depends on endosomal membrane phosphoinositides (PtdIns) synthesized by PIKfyve. Endosomes could act as platforms providing membranes and PtdIns, necessary for ASFV replication. Our study has revealed that ASFV reorganizes endosome dynamics, in order to ensure a productive infection. View Full-Text
Keywords: african swine fever virus (ASFV); ASF viral factory; endosomes; endosomal pathway; phosphoinositides; phosphatidylinositol kinases; microtubules african swine fever virus (ASFV); ASF viral factory; endosomes; endosomal pathway; phosphoinositides; phosphatidylinositol kinases; microtubules
Show Figures

Figure 1

MDPI and ACS Style

Cuesta-Geijo, M.Á.; Barrado-Gil, L.; Galindo, I.; Muñoz-Moreno, R.; Alonso, C. Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site. Viruses 2017, 9, 133.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop