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Viruses 2016, 8(5), 108;

Conserved Endonuclease Function of Hantavirus L Polymerase

Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne CH-1011, Switzerland
SIB Swiss Institute of Bioinformatics, Lausanne CH-1015, Switzerland
SPIEZ Laboratory, Austrasse, Spiez CH-3700, Switzerland
Authors to whom correspondence should be addressed.
Academic Editors: Jane Tao and Pierre-Yves Lozach
Received: 27 February 2016 / Revised: 18 April 2016 / Accepted: 19 April 2016 / Published: 2 May 2016
(This article belongs to the Special Issue Recent Progress in Bunyavirus Research)
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Hantaviruses are important emerging pathogens belonging to the Bunyaviridae family. Like other segmented negative strand RNA viruses, the RNA-dependent RNA polymerase (RdRp) also known as L protein of hantaviruses lacks an intrinsic “capping activity”. Hantaviruses therefore employ a “cap snatching” strategy acquiring short 5′ RNA sequences bearing 5′cap structures by endonucleolytic cleavage from host cell transcripts. The viral endonuclease activity implicated in cap snatching of hantaviruses has been mapped to the N-terminal domain of the L protein. Using a combination of molecular modeling and structure–function analysis we confirm and extend these findings providing evidence for high conservation of the L endonuclease between Old and New World hantaviruses. Recombinant hantavirus L endonuclease showed catalytic activity and a defined cation preference shared by other viral endonucleases. Based on the previously reported remarkably high activity of hantavirus L endonuclease, we established a cell-based assay for the hantavirus endonuclase function. The robustness of the assay and its high-throughput compatible format makes it suitable for small molecule drug screens to identify novel inhibitors of hantavirus endonuclease. Based on the high degree of similarity to RdRp endonucleases, some candidate inhibitors may be broadly active against hantaviruses and other emerging human pathogenic Bunyaviruses. View Full-Text
Keywords: Bunyaviridae; hantavirus; emerging diseases; endonuclease Bunyaviridae; hantavirus; emerging diseases; endonuclease

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Rothenberger, S.; Torriani, G.; Johansson, M.U.; Kunz, S.; Engler, O. Conserved Endonuclease Function of Hantavirus L Polymerase. Viruses 2016, 8, 108.

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