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Open AccessArticle

Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism

Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 225 Johnson Pavilion, 3610 HamiltonWalk, Philadelphia, PA 19104-6076, USA
Author to whom correspondence should be addressed.
Academic Editor: Jens H. Kuhn
Viruses 2015, 7(10), 5587-5602;
Received: 9 July 2015 / Revised: 6 October 2015 / Accepted: 14 October 2015 / Published: 26 October 2015
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
Tetherin is an interferon-induced, intrinsic cellular response factor that blocks release of numerous viruses, including Ebola virus, from infected cells. As with many viruses targeted by host factors, Ebola virus employs a tetherin antagonist, the viral glycoprotein (EboGP), to counteract restriction and promote virus release. Unlike other tetherin antagonists such as HIV-1 Vpu or KSHV K5, the features within EboGP needed to overcome tetherin are not well characterized. Here, we describe sequences within the EboGP ectodomain and membrane spanning domain (msd) as necessary to relieve tetherin restriction of viral particle budding. Fusing the EboGP msd to a normally secreted form of the glycoprotein effectively promotes Ebola virus particle release. Cellular protein or lipid anchors could not substitute for the EboGP msd. The requirement for the EboGP msd was not specific for filovirus budding, as similar results were seen with HIV particles. Furthermore trafficking of chimeric proteins to budding sites did not correlate with an ability to counter tetherin. Additionally, we find that a glycoprotein construct, which mimics the cathepsin-activated species by proteolytic removal of the EboGP glycan cap and mucin domains, is unable to counteract tetherin. Combining these results suggests an important role for the EboGP glycan cap and msd in tetherin antagonism. View Full-Text
Keywords: tetherin; BST2; Ebola virus; Ebola; glycoprotein; GP-primed; primed GP; sGP tetherin; BST2; Ebola virus; Ebola; glycoprotein; GP-primed; primed GP; sGP
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Vande Burgt, N.H.; Kaletsky, R.L.; Bates, P. Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism. Viruses 2015, 7, 5587-5602.

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