Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines, Plasmid Vectors and Antibodies
2.2. Virus-Like Particle (VLP) Budding Assay
2.3. Immunoprecipitaion Assay
2.4. Immunoblot Analysis
2.5. Flow Cytometry Analysis
3. Results
3.1. Requirements within EboGP for Tetherin Antagonism
3.2. Chimeras Reveal a Specific Requirement for the EboGP Membrane Spanning Domain (msd)
3.3. The Ebola msd Requirement is not Specific for Filoviral Budding
3.4. The Tetherin Amino-Terminal Region is not Required for EboGP Recognition
3.5. The Glycan Cap of EboGP is Required to Antagonize Tetherin
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Vande Burgt, N.H.; Kaletsky, R.L.; Bates, P. Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism. Viruses 2015, 7, 5587-5602. https://doi.org/10.3390/v7102888
Vande Burgt NH, Kaletsky RL, Bates P. Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism. Viruses. 2015; 7(10):5587-5602. https://doi.org/10.3390/v7102888
Chicago/Turabian StyleVande Burgt, Nathan H., Rachel L. Kaletsky, and Paul Bates. 2015. "Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism" Viruses 7, no. 10: 5587-5602. https://doi.org/10.3390/v7102888