Next Article in Journal
PAN’s Labyrinth: Molecular Biology of Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) PAN RNA, a Multifunctional Long Noncoding RNA
Previous Article in Journal
Epidemiology and Transmission of Kaposi’s Sarcoma-Associated Herpesvirus
Open AccessArticle

Cytoplasmic Dynein Promotes HIV-1 Uncoating

Laboratory of Retrovirology, Department of Medical Biology and BioMed Research Center, Université du Québec à Trois-Rivières, 3351 Boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada
*
Author to whom correspondence should be addressed.
Viruses 2014, 6(11), 4195-4211; https://doi.org/10.3390/v6114195
Received: 2 October 2014 / Revised: 24 October 2014 / Accepted: 27 October 2014 / Published: 4 November 2014
(This article belongs to the Section Animal Viruses)
Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role of dynein-dependent transport in HIV-1 uncoating. To interfere with dynein function, we depleted dynein heavy chain (DHC) using RNA interference, and we over-expressed p50/dynamitin. In immunofluorescence microscopy experiments, DHC depletion caused an accumulation of CA foci in HIV-1 infected cells. Using a biochemical assay to monitor HIV-1 CA core disassembly in infected cells, we observed an increase in amounts of intact (pelletable) CA cores upon DHC depletion or p50 over-expression. Results from these two complementary assays suggest that inhibiting dynein-mediated transport interferes with HIV-1 uncoating in infected cells, indicating the existence of a functional link between HIV-1 transport and uncoating. View Full-Text
Keywords: HIV-1; uncoating; capsid; microtubules; dynein HIV-1; uncoating; capsid; microtubules; dynein
Show Figures

Figure 1

MDPI and ACS Style

Pawlica, P.; Berthoux, L. Cytoplasmic Dynein Promotes HIV-1 Uncoating. Viruses 2014, 6, 4195-4211.

Show more citation formats Show less citations formats

Article Access Map

1
Only visits after 24 November 2015 are recorded.
Back to TopTop