Chronic bee paralysis virus (CBPV) is an important viral disease of adult bees which induces significant losses in honey bee colonies. Despite comprehensive research, only limited data is available from experimental infection for this virus. In the present study winter worker bees were experimentally infected in three different experiments. Bees were first inoculated per os (p/o
) or per cuticle (p/c
) with CBPV field strain M92/2010 in order to evaluate the virus replication in individual bees. In addition, potential synergistic effects of co-infection with CBPV and Nosema ceranae
) on bees were investigated. In total 558 individual bees were inoculated in small cages and data were analyzed using quantitative real time RT-PCR (RT-qPCR). Our results revealed successful replication of CBPV after p/o
inoculation, while it was less effective when bees were inoculated p/c
. Dead bees harbored about 1,000 times higher copy numbers of the virus than live bees. Co-infection of workers with CBPV and N. ceranae
using either method of virus inoculation
(p/c or p/o
increased replication ability for CBPV. In the third experiment the effect of inoculation on bee mortality was evaluated. The highest level of bee mortality was observed in a group of bees inoculated with CBPV p/o
, followed by a group of workers simultaneously inoculated with CBPV and N. ceranae p/o
, followed by the group inoculated with CBPV p/c
and the group with only N. ceranae p/o.
experimental infection with CBPV showed important differences after p/o
in winter bees, while simultaneous infection with
CBPV and N. ceranae
suggesting a synergistic effect after inoculation.