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Article

A New Multiplex Real-Time RT-PCR for Simultaneous Detection and Differentiation of Avian Bornaviruses

1
Department of Poultry and Rabbit Diseases, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
2
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald, Insel Riems, Germany
3
Medical Center, Institute of Virology, University of Freiburg, 79104 Freiburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Ian N. Hampson
Viruses 2021, 13(7), 1358; https://doi.org/10.3390/v13071358
Received: 27 May 2021 / Revised: 8 July 2021 / Accepted: 9 July 2021 / Published: 13 July 2021
(This article belongs to the Section Animal Viruses)
Avian bornaviruses were first described in 2008 as the causative agents of proventricular dilatation disease (PDD) in parrots and their relatives (Psittaciformes). To date, 15 genetically highly diverse avian bornaviruses covering at least five viral species have been discovered in different bird orders. Currently, the primary diagnostic tool is the detection of viral RNA by conventional or real-time RT-PCR (rRT-PCR). One of the drawbacks of this is the usage of either specific assays, allowing the detection of one particular virus, or of assays with a broad detection spectrum, which, however, do not allow for the simultaneous specification of the detected virus. To facilitate the simultaneous detection and specification of avian bornaviruses, a multiplex real-time RT-PCR assay was developed. Whole-genome sequences of various bornaviruses were aligned. Primers were designed to recognize conserved regions within the overlapping X/P gene and probes were selected to detect virus species-specific regions within the target region. The optimization of the assay resulted in the sensitive and specific detection of bornaviruses of Psittaciformes, Passeriformes, and aquatic birds. Finally, the new rRT-PCR was successfully employed to detect avian bornaviruses in field samples from various avian species. This assay will serve as powerful tool in epidemiological studies and will improve avian bornavirus detection. View Full-Text
Keywords: avian bornavirus; diagnostics; multiplex real-time RT-PCR avian bornavirus; diagnostics; multiplex real-time RT-PCR
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MDPI and ACS Style

Sigrist, B.; Geers, J.; Albini, S.; Rubbenstroth, D.; Wolfrum, N. A New Multiplex Real-Time RT-PCR for Simultaneous Detection and Differentiation of Avian Bornaviruses. Viruses 2021, 13, 1358. https://doi.org/10.3390/v13071358

AMA Style

Sigrist B, Geers J, Albini S, Rubbenstroth D, Wolfrum N. A New Multiplex Real-Time RT-PCR for Simultaneous Detection and Differentiation of Avian Bornaviruses. Viruses. 2021; 13(7):1358. https://doi.org/10.3390/v13071358

Chicago/Turabian Style

Sigrist, Brigitte, Jessica Geers, Sarah Albini, Dennis Rubbenstroth, and Nina Wolfrum. 2021. "A New Multiplex Real-Time RT-PCR for Simultaneous Detection and Differentiation of Avian Bornaviruses" Viruses 13, no. 7: 1358. https://doi.org/10.3390/v13071358

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