HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid Nanoparticles
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Liposomes (LPs)
2.4. Confocal Laser Microscopy
2.5. Flow Cytometry
2.6. Alphascreen® Assay
2.7. Bio-Layer-Interferometry (BLI) Analysis
3. Results
3.1. Inhibition of Cellular Uptake of Liposomes (LPs) by Myr47
3.2. N-Myristoylation-Dependent Interaction between Myr47 and LPs
3.3. Effect of Amino Acid Sequence on the Myr47-Mediated Inhibitory Activity
3.4. Effect of Myr47 on the ApoE3 (Apolipoprotein E3)-LPs Interaction
3.5. Effect of Myr47 on the Cellular Uptake of HBsAg
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
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Name | Sequence |
---|---|
Myr47 | Myr-GTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDQWPEANQVK-biotin |
aa2–48 | GTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDQWPEANQVK-biotin |
Ala11–15 | Myr-GTNLSVPNPAAAAADHQLDPAFGANSNNPDWDFNPNKDQWPEANQVK-biotin |
d-11,13 | Myr-GTNLSVPNLFFPDHQLDPAFGANSNNPDWDFNPNKDQWPEANQVK-biotin |
Scrambled (Scr) | Myr-TNNDPFKRTDLAWNGFDSVAQNDLLPNPPFNNWGDGSHQPADAKPFTK-biotin |
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Nanahara, M.; Chang, Y.-T.; Somiya, M.; Kuroda, S. HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid Nanoparticles. Viruses 2021, 13, 929. https://doi.org/10.3390/v13050929
Nanahara M, Chang Y-T, Somiya M, Kuroda S. HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid Nanoparticles. Viruses. 2021; 13(5):929. https://doi.org/10.3390/v13050929
Chicago/Turabian StyleNanahara, Masaya, Ya-Ting Chang, Masaharu Somiya, and Shun’ichi Kuroda. 2021. "HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid Nanoparticles" Viruses 13, no. 5: 929. https://doi.org/10.3390/v13050929
APA StyleNanahara, M., Chang, Y.-T., Somiya, M., & Kuroda, S. (2021). HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid Nanoparticles. Viruses, 13(5), 929. https://doi.org/10.3390/v13050929