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Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season

1
Department of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, Russia
2
Children’s City Hospital of St. Olga, 194017 Saint-Petersburg, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Oliver Schildgen
Viruses 2021, 13(1), 119; https://doi.org/10.3390/v13010119
Received: 16 November 2020 / Revised: 10 January 2021 / Accepted: 12 January 2021 / Published: 17 January 2021
(This article belongs to the Section Animal Viruses)
Human respiratory syncytial virus (RSV) is the most common cause of upper and lower respiratory tract infections in infants and young children. It is actively evolving under environmental and herd immunity influences. This work presents, for the first time, sequence variability analysis of RSV G gene and G protein using St. Petersburg (Russia) isolates. Viruses were isolated in a cell culture from the clinical samples of 61 children hospitalized (January–April 2014) with laboratory-confirmed RSV infection. Real-time RT-PCR data showed that 56 isolates (91.8%) belonged to RSV-A and 5 isolates (8.2%) belonged to RSV-B. The G genes were sequenced for 27 RSV-A isolates and all of them belonged to genotype ON1/GA2. Of these RSV-A, 77.8% belonged to the ON1(1.1) genetic sub-cluster, and 14.8% belonged to the ON1(1.2) sub-cluster. The ON1(1.3) sub-cluster constituted a minor group (3.7%). Many single-amino acid substitutions were identified in the G proteins of St. Petersburg isolates, compared with the Canadian ON1/GA2 reference virus (ON67-1210A). Most of the amino acid replacements were found in immunodominant B- and T-cell antigenic determinants of G protein. These may affect the antigenic characteristics of RSV and influence the host antiviral immune response to currently circulating viruses. View Full-Text
Keywords: human respiratory syncytial virus; phylogenetic analysis; ON1/GA2 genotype; G protein variability human respiratory syncytial virus; phylogenetic analysis; ON1/GA2 genotype; G protein variability
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MDPI and ACS Style

Krivitskaya, V.; Komissarova, K.; Pisareva, M.; Sverlova, M.; Fadeev, A.; Petrova, E.; Timonina, V.; Sominina, A.; Danilenko, D. Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season. Viruses 2021, 13, 119. https://doi.org/10.3390/v13010119

AMA Style

Krivitskaya V, Komissarova K, Pisareva M, Sverlova M, Fadeev A, Petrova E, Timonina V, Sominina A, Danilenko D. Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season. Viruses. 2021; 13(1):119. https://doi.org/10.3390/v13010119

Chicago/Turabian Style

Krivitskaya, Vera, Kseniya Komissarova, Maria Pisareva, Maria Sverlova, Artem Fadeev, Ekaterina Petrova, Veronika Timonina, Anna Sominina, and Daria Danilenko. 2021. "Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season" Viruses 13, no. 1: 119. https://doi.org/10.3390/v13010119

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