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Article

Changes in MicroRNA Expression during Rabbit Hemorrhagic Disease Virus (RHDV) Infection

1
Institute of Biology, University of Szczecin, 71-412 Szczecin, Poland
2
Molecular Biology and Biotechnology Center, University of Szczecin, 71-412 Szczecin, Poland
3
Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Warsaw, 02-091 Warsaw, Poland
4
Department of Medical Biology, Pomeranian Medical University, 70-204 Szczecin, Poland
5
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
6
Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(9), 965; https://doi.org/10.3390/v12090965
Received: 4 August 2020 / Revised: 26 August 2020 / Accepted: 30 August 2020 / Published: 31 August 2020
(This article belongs to the Section Animal Viruses)
Current knowledge on the role of microRNAs (miRNAs) in rabbit hemorrhagic disease virus (RHDV) infection and the pathogenesis of rabbit hemorrhagic disease (RHD) is still limited. RHDV replicates in the liver, causing hepatic necrosis and liver failure. MiRNAs are a class of short RNA molecules, and their expression profiles vary over the course of diseases, both in the tissue environment and in the bloodstream. This paper evaluates the expression of miRNAs in the liver tissue (ocu-miR-122-5p, ocu-miR-155-5p, and ocu-miR-16b-5p) and serum (ocu-miR-122-5p) of rabbits experimentally infected with RHDV. The expression levels of ocu-miR-122-5p, ocu-miR-155-5p, and ocu-miR-16b-5p in liver tissue were determined using reverse transcription quantitative real-time PCR (RT-qPCR), and the expression level of circulating ocu-miR-122-5p was established using droplet digital PCR (ddPCR). The expression levels of ocu-miR-155-5p and ocu-miR-16b-5p were significantly higher in the infected rabbits compared to the healthy rabbits (a fold-change of 5.8 and 2.5, respectively). The expression of ocu-miR-122-5p was not significantly different in the liver tissue from the infected rabbits compared to the healthy rabbits (p = 0.990), while the absolute expression level of the circulating ocu-miR-122-5p was significantly higher in the infected rabbits than in the healthy rabbits (p < 0.0001). Furthermore, a functional analysis showed that ocu-miR-155-5p, ocu-miR-16b-5p, and ocu-miR-122-5p can regulate the expression of genes involved in processes correlated with acute liver failure (ALF) in rabbits. Search tool for the retrieval of interacting genes/proteins (STRING) analysis showed that the potential target genes of the three selected miRNAs may interact with each other in different pathways. The results indicate the roles of these miRNAs in RHDV infection and over the course of RHD and may reflect hepatic inflammation and impairment/dysfunction in RHD. View Full-Text
Keywords: microRNA; rabbit hemorrhagic disease virus (RHDV); liver, STRING analysis microRNA; rabbit hemorrhagic disease virus (RHDV); liver, STRING analysis
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MDPI and ACS Style

Hukowska-Szematowicz, B.; Maciejak-Jastrzębska, A.; Blatkiewicz, M.; Maciak, K.; Góra, M.; Janiszewska, J.; Burzyńska, B. Changes in MicroRNA Expression during Rabbit Hemorrhagic Disease Virus (RHDV) Infection. Viruses 2020, 12, 965. https://doi.org/10.3390/v12090965

AMA Style

Hukowska-Szematowicz B, Maciejak-Jastrzębska A, Blatkiewicz M, Maciak K, Góra M, Janiszewska J, Burzyńska B. Changes in MicroRNA Expression during Rabbit Hemorrhagic Disease Virus (RHDV) Infection. Viruses. 2020; 12(9):965. https://doi.org/10.3390/v12090965

Chicago/Turabian Style

Hukowska-Szematowicz, Beata, Agata Maciejak-Jastrzębska, Małgorzata Blatkiewicz, Karolina Maciak, Monika Góra, Joanna Janiszewska, and Beata Burzyńska. 2020. "Changes in MicroRNA Expression during Rabbit Hemorrhagic Disease Virus (RHDV) Infection" Viruses 12, no. 9: 965. https://doi.org/10.3390/v12090965

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