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Article

Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

1
Faculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech Republic
2
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 České Budějovice, Czech Republic
3
Department of Virology, Veterinary Research Institute, 62100 Brno, Czech Republic
4
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UK
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(8), 902; https://doi.org/10.3390/v12080902
Received: 17 July 2020 / Revised: 10 August 2020 / Accepted: 13 August 2020 / Published: 18 August 2020
(This article belongs to the Section Animal Viruses)
A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and Ixodes ricinus tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals. View Full-Text
Keywords: TBEV; host alternation; neuroinvasiveness; genome mutation; quasispecies; flavivirus adaptation; tick cell line TBEV; host alternation; neuroinvasiveness; genome mutation; quasispecies; flavivirus adaptation; tick cell line
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MDPI and ACS Style

Helmová, R.; Hönig, V.; Tykalová, H.; Palus, M.; Bell-Sakyi, L.; Grubhoffer, L. Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies. Viruses 2020, 12, 902. https://doi.org/10.3390/v12080902

AMA Style

Helmová R, Hönig V, Tykalová H, Palus M, Bell-Sakyi L, Grubhoffer L. Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies. Viruses. 2020; 12(8):902. https://doi.org/10.3390/v12080902

Chicago/Turabian Style

Helmová, Renata, Václav Hönig, Hana Tykalová, Martin Palus, Lesley Bell-Sakyi, and Libor Grubhoffer. 2020. "Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies" Viruses 12, no. 8: 902. https://doi.org/10.3390/v12080902

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