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Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species

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School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK
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Chester Zoo, Chester, Cheshire CH2 1EU, UK
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Twycross Zoo, Atherstone, Warwickshire CV9 3PX, UK
4
School of Veterinary Science, University of Nottingham, Sutton Bonnington, Loughborough LE12 5RD, UK
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(4), 454; https://doi.org/10.3390/v12040454
Received: 26 February 2020 / Revised: 10 April 2020 / Accepted: 13 April 2020 / Published: 17 April 2020
(This article belongs to the Section Animal Viruses)
Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (Microtus agrestis) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus. View Full-Text
Keywords: Orthohantavirus; hantavirus; high-throughput sequencing; virus discovery; field vole; United Kingdom Orthohantavirus; hantavirus; high-throughput sequencing; virus discovery; field vole; United Kingdom
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Chappell, J.G.; Tsoleridis, T.; Onianwa, O.; Drake, G.; Ashpole, I.; Dobbs, P.; Edema, W.; Kumi-Ansah, F.; Bennett, M.; Tarlinton, R.E.; Ball, J.K.; McClure, C.P. Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species. Viruses 2020, 12, 454.

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