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Open AccessArticle

Metabolomic Analysis of Cricket paralysis virus Infection in Drosophila S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells

1
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
2
Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
3
Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, 153 10 Athens, Greece
4
Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(4), 393; https://doi.org/10.3390/v12040393
Received: 18 February 2020 / Revised: 6 March 2020 / Accepted: 31 March 2020 / Published: 1 April 2020
(This article belongs to the Section Insect Viruses)
High-throughput approaches have opened new opportunities for understanding biological processes such as persistent virus infections, which are widespread. However, the potential of persistent infections to develop towards pathogenesis remains to be investigated, particularly with respect to the role of host metabolism. To explore the interactions between cellular metabolism and persistent/pathogenic virus infection, we performed untargeted and targeted metabolomic analysis to examine the effects of Cricket paralysis virus (CrPV, Dicistroviridae) in persistently infected silkworm Bm5 cells and acutely infected Drosophila S2 cells. Our previous study (Viruses 2019, 11, 861) established that both glucose and glutamine levels significantly increased during the persistent period of CrPV infection of Bm5 cells, while they decreased steeply during the pathogenic stages. Strikingly, in this study, an almost opposite pattern in change of metabolites was observed during different stages of acute infection of S2 cells. More specifically, a significant decrease in amino acids and carbohydrates was observed prior to pathogenesis, while their abundance significantly increased again during pathogenesis. Our study illustrates the occurrence of diametrically opposite changes in central carbon mechanisms during CrPV infection of S2 and Bm5 cells that is possibly related to the type of infection (acute or persistent) that is triggered by the virus. View Full-Text
Keywords: metabolomics; persistent infection; host metabolism; Cricket paralysis virus; central carbon metabolism metabolomics; persistent infection; host metabolism; Cricket paralysis virus; central carbon metabolism
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MDPI and ACS Style

Wang, L.-L.; Swevers, L.; Van Meulebroek, L.; Meeus, I.; Vanhaecke, L.; Smagghe, G. Metabolomic Analysis of Cricket paralysis virus Infection in Drosophila S2 Cells Reveals Divergent Effects on Central Carbon Metabolism as Compared with Silkworm Bm5 Cells. Viruses 2020, 12, 393.

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