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Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of Haliotis diversicolor supertexta against Haliotid Herpesvirus-1

1
Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
2
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Department of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
4
Department of Fisheries, Tianjin Agriculture University, Tianjin 300380, China
5
Department of Biology, University of Padua, 35121 Padua, Italy
6
Alfred Wegener Institute (AWI)—Helmholtz Centre for Polar and Marine Research, Wadden Sea Station Sylt, 25992 List, Germany
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(4), 383; https://doi.org/10.3390/v11040383
Received: 25 February 2019 / Revised: 15 April 2019 / Accepted: 23 April 2019 / Published: 24 April 2019
(This article belongs to the Section Animal Viruses)
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Abstract

Haliotid herpesvirus-1 (HaHV-1) is the first identified gastropod herpesvirus, causing a highly lethal neurologic disease of abalone species. The genome of HaHV-1 has been sequenced, but the functions of the putative genes and their roles during infection are still poorly understood. In the present study, transcriptomic profiles of Haliotis diversicolor supertexta at 0, 24 and 60 h post injection (hpi) with HaHV-1 were characterized through high-throughput RNA sequencing. A total of 448 M raw reads were obtained and assembled into 2.08 × 105 unigenes with a mean length of 1486 bp and an N50 of 2455 bp. Although we detected increased HaHV-1 DNA loads and active viral expression at 24 hpi, this evidence was not linked to significant changes of host transcriptomic profiles between 0 and 24 hpi, whereas a rich immune-related gene set was over-expressed at 60 hpi. These results indicate that, at least at the beginning of HaHV-1 infection, the virus can replicate with no activation of the host immune response. We propose that HaHV-1 may evolve more effective strategies to modulate the host immune response and hide during replication, so that it could evade the immune surveillance at the early stage of infection. View Full-Text
Keywords: abalone; transcriptome; immune-related genes; apoptosis; HaHV-1 abalone; transcriptome; immune-related genes; apoptosis; HaHV-1
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Bai, C.-M.; Zhang, S.-M.; Li, Y.-N.; Xin, L.-S.; Rosani, U.; Wang, C.-M. Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of Haliotis diversicolor supertexta against Haliotid Herpesvirus-1. Viruses 2019, 11, 383.

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