Next Article in Journal
Mycoviruses: Past, Present, and Future
Next Article in Special Issue
Periplasmic Nanobody-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus
Previous Article in Journal
Feline Foamy Virus is Highly Prevalent in Free-Ranging Puma concolor from Colorado, Florida and Southern California
Previous Article in Special Issue
Mechanism of Inhibition of Ebola Virus RNA-Dependent RNA Polymerase by Remdesivir
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain

1
Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain
2
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA
3
Department of Pathology & Microbiology, University of Nebraska Medical Centre, Omaha, NE 68198, USA
4
National Centre of Microbiology, Parasitology Section, Majadahonda, 28220 Madrid, Spain
5
National Centre of Microbiology, Institute of Health Carlos III, Majadahonda, 28220 Madrid, Spain
6
National Centre of Microbiology, Viral Immunology Laboratory, Majadahonda, 28220 Madrid, Spain
7
Centro de Investigación en Sanidad Animal-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28040 Madrid, Spain
8
Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), 28029 Madrid, Spain
9
Museo de la Naturaleza de Cantabria, Carrejo, 39592 Cantabria, Spain
10
National Centre of Microbiology, Virology Section, Majadahonda, 28220 Madrid, Spain
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(4), 360; https://doi.org/10.3390/v11040360
Received: 19 February 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 19 April 2019
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
  |  
PDF [3861 KB, uploaded 19 April 2019]
  |     |  

Abstract

Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of the previously reported die-offs. View Full-Text
Keywords: Lloviu virus; Prevalence; Serology; Human; Bats Lloviu virus; Prevalence; Serology; Human; Bats
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Ramírez de Arellano, E.; Sanchez-Lockhart, M.; Perteguer, M.J.; Bartlett, M.; Ortiz, M.; Campioli, P.; Hernández, A.; Gonzalez, J.; Garcia, K.; Ramos, M.; Jiménez-Clavero, M.Á.; Tenorio, A.; Sánchez-Seco, M.P.; González, F.; Echevarría, J.E.; Palacios, G.; Negredo, A. First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain. Viruses 2019, 11, 360.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top