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First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain

Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA
Department of Pathology & Microbiology, University of Nebraska Medical Centre, Omaha, NE 68198, USA
National Centre of Microbiology, Parasitology Section, Majadahonda, 28220 Madrid, Spain
National Centre of Microbiology, Institute of Health Carlos III, Majadahonda, 28220 Madrid, Spain
National Centre of Microbiology, Viral Immunology Laboratory, Majadahonda, 28220 Madrid, Spain
Centro de Investigación en Sanidad Animal-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28040 Madrid, Spain
Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), 28029 Madrid, Spain
Museo de la Naturaleza de Cantabria, Carrejo, 39592 Cantabria, Spain
National Centre of Microbiology, Virology Section, Majadahonda, 28220 Madrid, Spain
Author to whom correspondence should be addressed.
Viruses 2019, 11(4), 360;
Received: 19 February 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 19 April 2019
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of the previously reported die-offs. View Full-Text
Keywords: Lloviu virus; Prevalence; Serology; Human; Bats Lloviu virus; Prevalence; Serology; Human; Bats
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Ramírez de Arellano, E.; Sanchez-Lockhart, M.; Perteguer, M.J.; Bartlett, M.; Ortiz, M.; Campioli, P.; Hernández, A.; Gonzalez, J.; Garcia, K.; Ramos, M.; Jiménez-Clavero, M.Á.; Tenorio, A.; Sánchez-Seco, M.P.; González, F.; Echevarría, J.E.; Palacios, G.; Negredo, A. First Evidence of Antibodies Against Lloviu Virus in Schreiber’s Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain. Viruses 2019, 11, 360.

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