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Viruses 2019, 11(3), 257; https://doi.org/10.3390/v11030257

The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)

1
State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Qingzhou Yatai Agricultural Technology Company Limited, Qingzhou 262500, China
3
Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
4
Fuzhou Entry-Exit Inspection & Quarantine Bureau of P.R.C, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Received: 19 January 2019 / Revised: 9 March 2019 / Accepted: 10 March 2019 / Published: 13 March 2019
(This article belongs to the Section Insect Viruses)
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Abstract

The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids. View Full-Text
Keywords: dimarhabdovirus; negative-sense (–) single-stranded RNA virus; viral genome; insect virus; phylogenetic analysis dimarhabdovirus; negative-sense (–) single-stranded RNA virus; viral genome; insect virus; phylogenetic analysis
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Meng, E.; Tang, B.; Sanchez-Garcia, F.J.; Qiao, T.; Fu, L.; Wang, Y.; Hou, Y.-M.; Wu, J.-L.; Chen, Z.-M. The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1). Viruses 2019, 11, 257.

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