The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
AbstractThe complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids. View Full-Text
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Meng, E.; Tang, B.; Sanchez-Garcia, F.J.; Qiao, T.; Fu, L.; Wang, Y.; Hou, Y.-M.; Wu, J.-L.; Chen, Z.-M. The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1). Viruses 2019, 11, 257.
Meng E, Tang B, Sanchez-Garcia FJ, Qiao T, Fu L, Wang Y, Hou Y-M, Wu J-L, Chen Z-M. The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1). Viruses. 2019; 11(3):257.Chicago/Turabian Style
Meng, E; Tang, Baozhen; Sanchez-Garcia, Francisco J.; Qiao, Ting; Fu, Lang; Wang, Yu; Hou, You-Ming; Wu, Jiang-Lin; Chen, Zhi-Ming. 2019. "The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)." Viruses 11, no. 3: 257.
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