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Forests 2017, 8(8), 279; https://doi.org/10.3390/f8080279

Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus

1
Centro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), Rua Pedro Soares, s.n.—Campus IPBeja/ESAB, Apartado 6158, 7801-908 Beja, Portugal
2
Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, Núcleo da Mitra, Apartado 94, 7006-554 Évora, Portugal
3
Instituto Nacional de Investigação Agrária e Veterinária, I.P. (INIAV), Avenida da República, Quinta do Marquês (edifício sede), 2780-157 Oeiras, Portugal
4
Faculdade de Ciências da Universidade de Lisboa (FCUL), BioSystems & Integrative Sciences Institute (BIOISI), Campo Grande, 1749-016 Lisboa, Portugal
5
Faculdade de Ciências da Universidade de Lisboa (FCUL), LaSIGE, Campo Grande, 1749-016 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Received: 22 June 2017 / Revised: 20 July 2017 / Accepted: 31 July 2017 / Published: 3 August 2017
(This article belongs to the Special Issue Genetics and Genomics of Forest Trees)
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Abstract

Forests are essential resources on a global scale, not only for the ecological benefits, but also for economical and landscape purposes. However, in recent years, a large number of forest species have suffered a serious decline, with maritime pine being one of the most affected. In Portugal, the maritime pine forest has been devastated by the pine wood nematode (PWN), the causal agent of pine wilt disease. In this study, RNA-Seq data was used to characterize the maritime pine response to infection with PWN, by determining the differentially expressed genes and identifying the regulatory networks and pathways associated. The analyses showed clear differences between an early response that occurs immediately after inoculation and a late response that is observed seven days after inoculation. Moreover, differentially expressed genes related to secondary metabolism, oxidative stress and defense against pathogen infection were identified over different time points. These results provide new insights about the molecular mechanisms and metabolic pathways involved in the response of Pinus pinaster against PWN infection, which will be a useful resource in follow-up studies and for future breeding programs to select plants with lower susceptibility to this disease. View Full-Text
Keywords: RNA-sequencing; transcriptome analysis; gene expression; pine wilt disease; Pinus pinaster; Bursaphelenchus xylophilus RNA-sequencing; transcriptome analysis; gene expression; pine wilt disease; Pinus pinaster; Bursaphelenchus xylophilus
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Gaspar, D.; Trindade, C.; Usié, A.; Meireles, B.; Barbosa, P.; Fortes, A.M.; Pesquita, C.; Costa, R.L.; Ramos, A.M. Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus. Forests 2017, 8, 279.

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