Next Article in Journal
Wood or Laminate?—Psychological Research of Customer Expectations
Next Article in Special Issue
Perceived Acceptability of Implementing Marker-Assisted Selection in the Forests of British Columbia
Previous Article in Journal
Optimization Forest Thinning Measures for Carbon Budget in a Mixed Pine-Oak Stand of the Qingling Mountains, China: A Case Study
Previous Article in Special Issue
Thousand Cankers Disease Complex: A Forest Health Issue that Threatens Juglans Species across the U.S.
Open AccessArticle

Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

1
Departamento de Ecologıía y Genética Forestal, INIA-CIFOR, Unidad Mixta de Genómica y Ecofisiología Forestal, INIA/UPM, Madrid 28040, Spain
2
Departamento de Biología Vegetal, Facultad de Farmacia, ERI BiotecMed, Universidad de Valencia, Burjassot, Valencia 46100, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: John MacKay and Stephen P. DiFazio
Forests 2016, 7(11), 274; https://doi.org/10.3390/f7110274
Received: 4 September 2016 / Revised: 4 November 2016 / Accepted: 9 November 2016 / Published: 12 November 2016
Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms. View Full-Text
Keywords: genetic stability; genome sequencing; haploid tissue; molecular markers; Pinus pinaster genetic stability; genome sequencing; haploid tissue; molecular markers; Pinus pinaster
Show Figures

Figure 1

MDPI and ACS Style

Cabezas, J.A.; Morcillo, M.; Vélez, M.D.; Díaz, L.; Segura, J.; Cervera, M.T.; Arrillaga, I. Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line. Forests 2016, 7, 274.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop